Age-specific locomotor response to nicotine in yellow and mottled yellow A§ssup§vy§esup§/a mice

Marc A. Dingman, Joseph P. Gyekis, Courtney A. Whetzel, Laura Klein, David John Vandenbergh

Research output: Contribution to journalArticle

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Abstract

Abstract. Background: Most Agouti viable yellow (A§ssup§vy§ esup§) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of excessive agouti expression on open-field locomotor response to daily 0.5 mg/kg (-)-freebase nicotine injections in 27 early adolescent and 27 young adult male A§ssup§vy§esup§/a and a/a mice, and assessed the effects of nicotine administration (0.5 mg/kg) followed by open-field testing on serum corticosterone levels in a separate group of 25 young adult male A§ssup§vy§esup§/a and a/a mice. Findings. Young adult A§ssup§vy§esup§/a mice displayed pronounced nicotine-induced hypolocomotion (a 24% reduction in distance traveled) compared to their a/a littermates. Early adolescent A§ssup§vy§esup§/a mice did not differ from their a/a littermates or saline-matched controls in locomotion following nicotine administration. Young adult A§ssup§vy§esup§/a mice also displayed increased thigmotaxis (a 5% increase in time spent outside the center of the apparatus) on the first day of nicotine administration as compared to saline-matched controls, while a/a mice did not. An increase in serum corticosterone levels 20 minutes after nicotine injection in a separate group of young adult male mice (n = 25) was proportionally similar between A§ssup§vy§esup§/a and a/a mice. Conclusions: Overall, the results suggest an age- and epigenotype- or genotype-specific response to nicotine administration in young adult male A§ssup§vy§esup§/ a mice. It appears the A§ssup§vy§esup§/a locomotor and thigmotaxic responses to acute nicotine administration are not mediated solely by hypothalamic-pituitary-adrenal (HPA) axis stimulation.

Original languageEnglish (US)
Article number497
JournalBMC Research Notes
Volume6
Issue number1
DOIs
StatePublished - Dec 1 2013

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Nicotine
Young Adult
Corticosterone
Agouti Signaling Protein
Receptor, Melanocortin, Type 4
Street Drugs
Injections
Locomotion
Serum
Obesity
Genotype
Testing

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{932c345d86ee4164b815f275ba261395,
title = "Age-specific locomotor response to nicotine in yellow and mottled yellow A§ssup§vy§esup§/a mice",
abstract = "Abstract. Background: Most Agouti viable yellow (A§ssup§vy§ esup§) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of excessive agouti expression on open-field locomotor response to daily 0.5 mg/kg (-)-freebase nicotine injections in 27 early adolescent and 27 young adult male A§ssup§vy§esup§/a and a/a mice, and assessed the effects of nicotine administration (0.5 mg/kg) followed by open-field testing on serum corticosterone levels in a separate group of 25 young adult male A§ssup§vy§esup§/a and a/a mice. Findings. Young adult A§ssup§vy§esup§/a mice displayed pronounced nicotine-induced hypolocomotion (a 24{\%} reduction in distance traveled) compared to their a/a littermates. Early adolescent A§ssup§vy§esup§/a mice did not differ from their a/a littermates or saline-matched controls in locomotion following nicotine administration. Young adult A§ssup§vy§esup§/a mice also displayed increased thigmotaxis (a 5{\%} increase in time spent outside the center of the apparatus) on the first day of nicotine administration as compared to saline-matched controls, while a/a mice did not. An increase in serum corticosterone levels 20 minutes after nicotine injection in a separate group of young adult male mice (n = 25) was proportionally similar between A§ssup§vy§esup§/a and a/a mice. Conclusions: Overall, the results suggest an age- and epigenotype- or genotype-specific response to nicotine administration in young adult male A§ssup§vy§esup§/ a mice. It appears the A§ssup§vy§esup§/a locomotor and thigmotaxic responses to acute nicotine administration are not mediated solely by hypothalamic-pituitary-adrenal (HPA) axis stimulation.",
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Age-specific locomotor response to nicotine in yellow and mottled yellow A§ssup§vy§esup§/a mice. / Dingman, Marc A.; Gyekis, Joseph P.; Whetzel, Courtney A.; Klein, Laura; Vandenbergh, David John.

In: BMC Research Notes, Vol. 6, No. 1, 497, 01.12.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Age-specific locomotor response to nicotine in yellow and mottled yellow A§ssup§vy§esup§/a mice

AU - Dingman, Marc A.

AU - Gyekis, Joseph P.

AU - Whetzel, Courtney A.

AU - Klein, Laura

AU - Vandenbergh, David John

PY - 2013/12/1

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N2 - Abstract. Background: Most Agouti viable yellow (A§ssup§vy§ esup§) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of excessive agouti expression on open-field locomotor response to daily 0.5 mg/kg (-)-freebase nicotine injections in 27 early adolescent and 27 young adult male A§ssup§vy§esup§/a and a/a mice, and assessed the effects of nicotine administration (0.5 mg/kg) followed by open-field testing on serum corticosterone levels in a separate group of 25 young adult male A§ssup§vy§esup§/a and a/a mice. Findings. Young adult A§ssup§vy§esup§/a mice displayed pronounced nicotine-induced hypolocomotion (a 24% reduction in distance traveled) compared to their a/a littermates. Early adolescent A§ssup§vy§esup§/a mice did not differ from their a/a littermates or saline-matched controls in locomotion following nicotine administration. Young adult A§ssup§vy§esup§/a mice also displayed increased thigmotaxis (a 5% increase in time spent outside the center of the apparatus) on the first day of nicotine administration as compared to saline-matched controls, while a/a mice did not. An increase in serum corticosterone levels 20 minutes after nicotine injection in a separate group of young adult male mice (n = 25) was proportionally similar between A§ssup§vy§esup§/a and a/a mice. Conclusions: Overall, the results suggest an age- and epigenotype- or genotype-specific response to nicotine administration in young adult male A§ssup§vy§esup§/ a mice. It appears the A§ssup§vy§esup§/a locomotor and thigmotaxic responses to acute nicotine administration are not mediated solely by hypothalamic-pituitary-adrenal (HPA) axis stimulation.

AB - Abstract. Background: Most Agouti viable yellow (A§ssup§vy§ esup§) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of excessive agouti expression on open-field locomotor response to daily 0.5 mg/kg (-)-freebase nicotine injections in 27 early adolescent and 27 young adult male A§ssup§vy§esup§/a and a/a mice, and assessed the effects of nicotine administration (0.5 mg/kg) followed by open-field testing on serum corticosterone levels in a separate group of 25 young adult male A§ssup§vy§esup§/a and a/a mice. Findings. Young adult A§ssup§vy§esup§/a mice displayed pronounced nicotine-induced hypolocomotion (a 24% reduction in distance traveled) compared to their a/a littermates. Early adolescent A§ssup§vy§esup§/a mice did not differ from their a/a littermates or saline-matched controls in locomotion following nicotine administration. Young adult A§ssup§vy§esup§/a mice also displayed increased thigmotaxis (a 5% increase in time spent outside the center of the apparatus) on the first day of nicotine administration as compared to saline-matched controls, while a/a mice did not. An increase in serum corticosterone levels 20 minutes after nicotine injection in a separate group of young adult male mice (n = 25) was proportionally similar between A§ssup§vy§esup§/a and a/a mice. Conclusions: Overall, the results suggest an age- and epigenotype- or genotype-specific response to nicotine administration in young adult male A§ssup§vy§esup§/ a mice. It appears the A§ssup§vy§esup§/a locomotor and thigmotaxic responses to acute nicotine administration are not mediated solely by hypothalamic-pituitary-adrenal (HPA) axis stimulation.

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