Aging mice show impaired memory updating in the novel OUL updating paradigm

Janine Kwapis, Yasaman Alaghband, Ashley A. Keiser, Tri N. Dong, Christina M. Michael, Diane Rhee, Guanhua Shu, Richard T. Dang, Dina P. Matheos, Marcelo A. Wood

Research output: Contribution to journalArticle

Abstract

Memories do not persist in a permanent, static state but instead must be dynamically modified in response to new information. Although new memory formation is typically studied in a laboratory setting, most real-world associations are modifications to existing memories, particularly in the aging, experienced brain. To date, the field has lacked a simple behavioral paradigm that can measure whether original and updated information is remembered in a single test session. To address this gap, we have developed a novel memory updating paradigm, called the Objects in Updated Locations (OUL) task that is capable of assessing memory updating in a non-stressful task that is appropriate for both young and old rodents. We first show that young mice successfully remember both the original memory and the updated information in OUL. Next, we demonstrate that intrahippocampal infusion of the protein synthesis inhibitor anisomycin disrupts both the updated information and the original memory at test, suggesting that memory updating in OUL engages the original memory. To verify this, we used the Arc CatFISH technique to show that the OUL update session reactivates a largely overlapping set of neurons as the original memory. Finally, using OUL, we show that memory updating is impaired in aging, 18-m.o. mice. Together, these results demonstrate that hippocampal memory updating is impaired with aging and establish that the OUL paradigm is an effective, sensitive method of assessing memory updating in rodents.

Original languageEnglish (US)
JournalNeuropsychopharmacology
DOIs
StatePublished - Jan 1 2019

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Rodentia
Anisomycin
Protein Synthesis Inhibitors
Neurons
Brain

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Kwapis, Janine ; Alaghband, Yasaman ; Keiser, Ashley A. ; Dong, Tri N. ; Michael, Christina M. ; Rhee, Diane ; Shu, Guanhua ; Dang, Richard T. ; Matheos, Dina P. ; Wood, Marcelo A. / Aging mice show impaired memory updating in the novel OUL updating paradigm. In: Neuropsychopharmacology. 2019.
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abstract = "Memories do not persist in a permanent, static state but instead must be dynamically modified in response to new information. Although new memory formation is typically studied in a laboratory setting, most real-world associations are modifications to existing memories, particularly in the aging, experienced brain. To date, the field has lacked a simple behavioral paradigm that can measure whether original and updated information is remembered in a single test session. To address this gap, we have developed a novel memory updating paradigm, called the Objects in Updated Locations (OUL) task that is capable of assessing memory updating in a non-stressful task that is appropriate for both young and old rodents. We first show that young mice successfully remember both the original memory and the updated information in OUL. Next, we demonstrate that intrahippocampal infusion of the protein synthesis inhibitor anisomycin disrupts both the updated information and the original memory at test, suggesting that memory updating in OUL engages the original memory. To verify this, we used the Arc CatFISH technique to show that the OUL update session reactivates a largely overlapping set of neurons as the original memory. Finally, using OUL, we show that memory updating is impaired in aging, 18-m.o. mice. Together, these results demonstrate that hippocampal memory updating is impaired with aging and establish that the OUL paradigm is an effective, sensitive method of assessing memory updating in rodents.",
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Kwapis, J, Alaghband, Y, Keiser, AA, Dong, TN, Michael, CM, Rhee, D, Shu, G, Dang, RT, Matheos, DP & Wood, MA 2019, 'Aging mice show impaired memory updating in the novel OUL updating paradigm', Neuropsychopharmacology. https://doi.org/10.1038/s41386-019-0438-0

Aging mice show impaired memory updating in the novel OUL updating paradigm. / Kwapis, Janine; Alaghband, Yasaman; Keiser, Ashley A.; Dong, Tri N.; Michael, Christina M.; Rhee, Diane; Shu, Guanhua; Dang, Richard T.; Matheos, Dina P.; Wood, Marcelo A.

In: Neuropsychopharmacology, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Kwapis, Janine

AU - Alaghband, Yasaman

AU - Keiser, Ashley A.

AU - Dong, Tri N.

AU - Michael, Christina M.

AU - Rhee, Diane

AU - Shu, Guanhua

AU - Dang, Richard T.

AU - Matheos, Dina P.

AU - Wood, Marcelo A.

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