Alcohol Reward Is Increased after Roux-en-Y Gastric Bypass in Dietary Obese Rats with Differential Effects following Ghrelin Antagonism

Andras Hajnal, Alevtina Zharikov, James E. Polston, Maxine R. Fields, Jonathan Tomasko, Ann Rogers, Nora D. Volkow, Panayotis K. Thanos

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Roux-en-Y gastric bypass (RYGB) is one of the most successful treatments for severe obesity and associated comorbidities. One potential adverse outcome, however, is increased risk for alcohol use. As such, we tested whether RYGB alters motivation to self-administer alcohol in outbred dietary obese rats, and investigated the involvement of the ghrelin system as a potential underlying mechanism. High fat (60%kcal from fat) diet-induced obese, non-diabetic male Sprague Dawley rats underwent RYGB (n = 9) or sham operation (Sham, n = 9) and were tested 4 months after surgery on a progressive ratio-10 (PR10) schedule of reinforcement operant task for 2, 4, and 8% ethanol. In addition, the effects of the ghrelin-1a-receptor antagonist D-[Lys3]-GHRP-6 (50, 100 nmol/kg, IP) were tested on PR10 responding for 4% ethanol. Compared to Sham, RYGB rats made significantly more active spout responses to earn reward, more consummatory licks on the ethanol spout, and achieved higher breakpoints. Pretreatment with a single peripheral injection of D-[Lys3]-GHRP-6 at either dose was ineffective in altering appetitive or consummatory responses to 4% ethanol in the Sham group. In contrast, RYGB rats demonstrated reduced operant performance to earn alcohol reward on the test day and reduced consummatory responses for two subsequent days following the drug. Sensitivity to threshold doses of D-[LYS3]-GHRP-6 suggests that an augmented ghrelin system may contribute to increased alcohol reward in RYGB. Further research is warranted to confirm applicability of these findings to humans and to explore ghrelin-receptor targets for treatment of alcohol-related disorders in RYGB patients.

Original languageEnglish (US)
Article numbere49121
JournalPloS one
Volume7
Issue number11
DOIs
StatePublished - Nov 7 2012

Fingerprint

bariatric surgery
ghrelin
Ghrelin
Gastric Bypass
Reward
Rats
alcohols
Alcohols
Ethanol
rats
ethanol
Ghrelin Receptor
Fats
Alcohol-Related Disorders
Nutrition
Surgery
Reinforcement Schedule
Morbid Obesity
Reinforcement
lipids

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hajnal, Andras ; Zharikov, Alevtina ; Polston, James E. ; Fields, Maxine R. ; Tomasko, Jonathan ; Rogers, Ann ; Volkow, Nora D. ; Thanos, Panayotis K. / Alcohol Reward Is Increased after Roux-en-Y Gastric Bypass in Dietary Obese Rats with Differential Effects following Ghrelin Antagonism. In: PloS one. 2012 ; Vol. 7, No. 11.
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abstract = "Roux-en-Y gastric bypass (RYGB) is one of the most successful treatments for severe obesity and associated comorbidities. One potential adverse outcome, however, is increased risk for alcohol use. As such, we tested whether RYGB alters motivation to self-administer alcohol in outbred dietary obese rats, and investigated the involvement of the ghrelin system as a potential underlying mechanism. High fat (60{\%}kcal from fat) diet-induced obese, non-diabetic male Sprague Dawley rats underwent RYGB (n = 9) or sham operation (Sham, n = 9) and were tested 4 months after surgery on a progressive ratio-10 (PR10) schedule of reinforcement operant task for 2, 4, and 8{\%} ethanol. In addition, the effects of the ghrelin-1a-receptor antagonist D-[Lys3]-GHRP-6 (50, 100 nmol/kg, IP) were tested on PR10 responding for 4{\%} ethanol. Compared to Sham, RYGB rats made significantly more active spout responses to earn reward, more consummatory licks on the ethanol spout, and achieved higher breakpoints. Pretreatment with a single peripheral injection of D-[Lys3]-GHRP-6 at either dose was ineffective in altering appetitive or consummatory responses to 4{\%} ethanol in the Sham group. In contrast, RYGB rats demonstrated reduced operant performance to earn alcohol reward on the test day and reduced consummatory responses for two subsequent days following the drug. Sensitivity to threshold doses of D-[LYS3]-GHRP-6 suggests that an augmented ghrelin system may contribute to increased alcohol reward in RYGB. Further research is warranted to confirm applicability of these findings to humans and to explore ghrelin-receptor targets for treatment of alcohol-related disorders in RYGB patients.",
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Alcohol Reward Is Increased after Roux-en-Y Gastric Bypass in Dietary Obese Rats with Differential Effects following Ghrelin Antagonism. / Hajnal, Andras; Zharikov, Alevtina; Polston, James E.; Fields, Maxine R.; Tomasko, Jonathan; Rogers, Ann; Volkow, Nora D.; Thanos, Panayotis K.

In: PloS one, Vol. 7, No. 11, e49121, 07.11.2012.

Research output: Contribution to journalArticle

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AU - Hajnal, Andras

AU - Zharikov, Alevtina

AU - Polston, James E.

AU - Fields, Maxine R.

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AU - Thanos, Panayotis K.

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