Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children

C. A. Contreras, T. J. Ochoa, D. W. Lacher, C. DebRoy, A. Navarro, M. Talledo, M. S. Donnenberg, L. Ecker, A. I. Gil, C. F. Lanata, T. G. Cleary

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Abstract

Enteropathogenic Escherichia coli (EPEC) is a leading cause of infantile diarrhoea in developing countries. The aim of this study was to describe the allelic diversity of critical EPEC virulence genes and their association with clinical characteristics. One hundred and twenty EPEC strains isolated from a cohort diarrhoea study in Peruvian children were characterized for the allele type of eae (intimin), bfpA (bundlin pilin protein of bundle-forming pilus) and perA (plasmid encoded regulator) genes by PCR-RFLP. Atypical EPEC strains (eae+, bfp") were the most common pathotype in diarrhoea (54/74, 73 %) and control samples from children without diarrhoea (40/46, 87 %). Overall, there were 13 eae alleles; the most common were beta (34/120, 28 %), theta (24/ 120, 20 %), kappa (14/120, 12 %) and mu (8/120, 7 %). There were five bfpA alleles; the most common were beta1/7 (10/26), alpha3 (7/26) and beta5 (3/26). There were three perA alleles: beta (8/16), alpha (7/16) and gamma (1/16). The strains belonged to 36 distinct serogroups; O55 was the most frequent. The gamma-intimin allele was more frequently found in diarrhoea episodes of longer duration (>7 days) than those of shorter duration (3/26, 12% vs 0/48, 0%, P<0.05). The kappa-intimin allele had the highest clinical severity score in comparison with other alleles (P<0.05). In Peruvian children, the virulence genes of EPEC strains are highly variable. Further studies are needed to evaluate additional virulence markers to determine whether relationships exist between specific variants and clinical features of disease.

Original languageEnglish (US)
Pages (from-to)25-31
Number of pages7
JournalJournal of Medical Microbiology
Volume59
Issue number1
DOIs
StatePublished - Jan 1 2010

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Enteropathogenic Escherichia coli
Virulence
Plasmids
Alleles
Diarrhea
Genes
Infantile Diarrhea
Fimbriae Proteins
Regulator Genes
Restriction Fragment Length Polymorphisms
Developing Countries
Cohort Studies
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Contreras, C. A. ; Ochoa, T. J. ; Lacher, D. W. ; DebRoy, C. ; Navarro, A. ; Talledo, M. ; Donnenberg, M. S. ; Ecker, L. ; Gil, A. I. ; Lanata, C. F. ; Cleary, T. G. / Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children. In: Journal of Medical Microbiology. 2010 ; Vol. 59, No. 1. pp. 25-31.
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title = "Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children",
abstract = "Enteropathogenic Escherichia coli (EPEC) is a leading cause of infantile diarrhoea in developing countries. The aim of this study was to describe the allelic diversity of critical EPEC virulence genes and their association with clinical characteristics. One hundred and twenty EPEC strains isolated from a cohort diarrhoea study in Peruvian children were characterized for the allele type of eae (intimin), bfpA (bundlin pilin protein of bundle-forming pilus) and perA (plasmid encoded regulator) genes by PCR-RFLP. Atypical EPEC strains (eae+, bfp{"}) were the most common pathotype in diarrhoea (54/74, 73 {\%}) and control samples from children without diarrhoea (40/46, 87 {\%}). Overall, there were 13 eae alleles; the most common were beta (34/120, 28 {\%}), theta (24/ 120, 20 {\%}), kappa (14/120, 12 {\%}) and mu (8/120, 7 {\%}). There were five bfpA alleles; the most common were beta1/7 (10/26), alpha3 (7/26) and beta5 (3/26). There were three perA alleles: beta (8/16), alpha (7/16) and gamma (1/16). The strains belonged to 36 distinct serogroups; O55 was the most frequent. The gamma-intimin allele was more frequently found in diarrhoea episodes of longer duration (>7 days) than those of shorter duration (3/26, 12{\%} vs 0/48, 0{\%}, P<0.05). The kappa-intimin allele had the highest clinical severity score in comparison with other alleles (P<0.05). In Peruvian children, the virulence genes of EPEC strains are highly variable. Further studies are needed to evaluate additional virulence markers to determine whether relationships exist between specific variants and clinical features of disease.",
author = "Contreras, {C. A.} and Ochoa, {T. J.} and Lacher, {D. W.} and C. DebRoy and A. Navarro and M. Talledo and Donnenberg, {M. S.} and L. Ecker and Gil, {A. I.} and Lanata, {C. F.} and Cleary, {T. G.}",
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Contreras, CA, Ochoa, TJ, Lacher, DW, DebRoy, C, Navarro, A, Talledo, M, Donnenberg, MS, Ecker, L, Gil, AI, Lanata, CF & Cleary, TG 2010, 'Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children', Journal of Medical Microbiology, vol. 59, no. 1, pp. 25-31. https://doi.org/10.1099/jmm.0.013706-0

Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children. / Contreras, C. A.; Ochoa, T. J.; Lacher, D. W.; DebRoy, C.; Navarro, A.; Talledo, M.; Donnenberg, M. S.; Ecker, L.; Gil, A. I.; Lanata, C. F.; Cleary, T. G.

In: Journal of Medical Microbiology, Vol. 59, No. 1, 01.01.2010, p. 25-31.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children

AU - Contreras, C. A.

AU - Ochoa, T. J.

AU - Lacher, D. W.

AU - DebRoy, C.

AU - Navarro, A.

AU - Talledo, M.

AU - Donnenberg, M. S.

AU - Ecker, L.

AU - Gil, A. I.

AU - Lanata, C. F.

AU - Cleary, T. G.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Enteropathogenic Escherichia coli (EPEC) is a leading cause of infantile diarrhoea in developing countries. The aim of this study was to describe the allelic diversity of critical EPEC virulence genes and their association with clinical characteristics. One hundred and twenty EPEC strains isolated from a cohort diarrhoea study in Peruvian children were characterized for the allele type of eae (intimin), bfpA (bundlin pilin protein of bundle-forming pilus) and perA (plasmid encoded regulator) genes by PCR-RFLP. Atypical EPEC strains (eae+, bfp") were the most common pathotype in diarrhoea (54/74, 73 %) and control samples from children without diarrhoea (40/46, 87 %). Overall, there were 13 eae alleles; the most common were beta (34/120, 28 %), theta (24/ 120, 20 %), kappa (14/120, 12 %) and mu (8/120, 7 %). There were five bfpA alleles; the most common were beta1/7 (10/26), alpha3 (7/26) and beta5 (3/26). There were three perA alleles: beta (8/16), alpha (7/16) and gamma (1/16). The strains belonged to 36 distinct serogroups; O55 was the most frequent. The gamma-intimin allele was more frequently found in diarrhoea episodes of longer duration (>7 days) than those of shorter duration (3/26, 12% vs 0/48, 0%, P<0.05). The kappa-intimin allele had the highest clinical severity score in comparison with other alleles (P<0.05). In Peruvian children, the virulence genes of EPEC strains are highly variable. Further studies are needed to evaluate additional virulence markers to determine whether relationships exist between specific variants and clinical features of disease.

AB - Enteropathogenic Escherichia coli (EPEC) is a leading cause of infantile diarrhoea in developing countries. The aim of this study was to describe the allelic diversity of critical EPEC virulence genes and their association with clinical characteristics. One hundred and twenty EPEC strains isolated from a cohort diarrhoea study in Peruvian children were characterized for the allele type of eae (intimin), bfpA (bundlin pilin protein of bundle-forming pilus) and perA (plasmid encoded regulator) genes by PCR-RFLP. Atypical EPEC strains (eae+, bfp") were the most common pathotype in diarrhoea (54/74, 73 %) and control samples from children without diarrhoea (40/46, 87 %). Overall, there were 13 eae alleles; the most common were beta (34/120, 28 %), theta (24/ 120, 20 %), kappa (14/120, 12 %) and mu (8/120, 7 %). There were five bfpA alleles; the most common were beta1/7 (10/26), alpha3 (7/26) and beta5 (3/26). There were three perA alleles: beta (8/16), alpha (7/16) and gamma (1/16). The strains belonged to 36 distinct serogroups; O55 was the most frequent. The gamma-intimin allele was more frequently found in diarrhoea episodes of longer duration (>7 days) than those of shorter duration (3/26, 12% vs 0/48, 0%, P<0.05). The kappa-intimin allele had the highest clinical severity score in comparison with other alleles (P<0.05). In Peruvian children, the virulence genes of EPEC strains are highly variable. Further studies are needed to evaluate additional virulence markers to determine whether relationships exist between specific variants and clinical features of disease.

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