Allosteric Effector ppGpp Potentiates the Inhibition of Transcript Initiation by DksA

Vadim Molodtsov, Elena Sineva, Lu Zhang, Xuhui Huang, Michael Cashel, Sarah E. Ades, Katsuhiko S. Murakami

20 Scopus citations

Abstract

DksA and ppGpp are the central players in the stringent response and mediate a complete reprogramming of the transcriptome. A major component of the response is a reduction in ribosome synthesis, which is accomplished by the synergistic action of DksA and ppGpp bound to RNA polymerase (RNAP) inhibiting transcription of rRNAs. Here, we report the X-ray crystal structures of Escherichia coli RNAP in complex with DksA alone and with ppGpp. The structures show that DksA accesses the template strand at the active site and the downstream DNA binding site of RNAP simultaneously and reveal that binding of the allosteric effector ppGpp reshapes the RNAP–DksA complex. The structural data support a model for transcriptional inhibition in which ppGpp potentiates the destabilization of open complexes by DksA. This work establishes a structural basis for understanding the pleiotropic effects of DksA and ppGpp on transcriptional regulation in proteobacteria. Molodtsov et al. provide structural analyses of the E. coli RNA polymerase holoenzyme complexes with DksA and TraR, which reveal a bimodal mechanism of DksA/TraR association with the enzyme. ppGpp assists DksA in transcriptional regulation by locking it in the functionally important mode. This study expands our understanding of synergistic mechanisms of transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)828-839.e5
JournalMolecular cell
Volume69
Issue number5
DOIs
StatePublished - Mar 1 2018

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