Alpha-subunit positions methionine 180 and glutamate 214 of Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase influence catalysis

Gönül Vardar, Thomas K. Wood

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.

Original languageEnglish (US)
Pages (from-to)1511-1514
Number of pages4
JournalJournal of bacteriology
Volume187
Issue number4
DOIs
StatePublished - Feb 2005

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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