Alterations in blood glucose levels under hyperinsulinemia affect accumbens dopamine

Nicholas T. Bello, Andras Hajnal

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Dopaminergic systems have been implicated in diabetes and obesity. Notwithstanding, the most basic relationship between dopamine and plasma insulin as well as glucose levels yet remains unknown. The present experiments were designed to investigate the effects of acute hyperinsulinemia on basal dopamine levels in the nucleus accumbens of the rat under chloral hydrate anesthesia using acute microdialysis in combination with the hyperinsulinemic-glycemic clamping procedure. In Experiment 1, each rat was infused with one of the three concentrations of insulin (2.4, 4.8, or 9.6 mU/kg per min) while plasma glucose levels were maintained at euglycemia (∼ 5.5 mmol/L). Dopamine, dihydroxyphenylacetic acid and homovanillic acid were not significantly different from baseline during either the clamp or post-clamp periods for all insulin concentrations. In Experiment 2, rats were infused with the highest concentration of insulin (9.6 mU/kg per min) and plasma glucose levels were maintained at either hypoglycemia (∼ 3 mmol/L) or hyperglycemia (∼ 14 mmol/L). Dopamine was elevated at 100 min (+ 113% above basal levels) and 120 min (+ 117%) in the hypoglycemic condition and at 120 min (+ 121%) in the hyperglycemic condition. In the hyperglycemic post-clamp period, homovanillic acid was decreased below basal levels (approximately - 32%). These results together suggest that short-term blood glucose deviations coupled with acute hyperinsulinemia affect the mesoaccumbens dopamine system.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalPhysiology and Behavior
Volume88
Issue number1-2
DOIs
StatePublished - Jun 15 2006

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Hyperinsulinism
Blood Glucose
Dopamine
Insulin
Homovanillic Acid
Glucose
Chloral Hydrate
Microdialysis
Nucleus Accumbens
Hypoglycemia
Hypoglycemic Agents
Constriction
Hyperglycemia
Anesthesia
Obesity
Acids

All Science Journal Classification (ASJC) codes

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

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abstract = "Dopaminergic systems have been implicated in diabetes and obesity. Notwithstanding, the most basic relationship between dopamine and plasma insulin as well as glucose levels yet remains unknown. The present experiments were designed to investigate the effects of acute hyperinsulinemia on basal dopamine levels in the nucleus accumbens of the rat under chloral hydrate anesthesia using acute microdialysis in combination with the hyperinsulinemic-glycemic clamping procedure. In Experiment 1, each rat was infused with one of the three concentrations of insulin (2.4, 4.8, or 9.6 mU/kg per min) while plasma glucose levels were maintained at euglycemia (∼ 5.5 mmol/L). Dopamine, dihydroxyphenylacetic acid and homovanillic acid were not significantly different from baseline during either the clamp or post-clamp periods for all insulin concentrations. In Experiment 2, rats were infused with the highest concentration of insulin (9.6 mU/kg per min) and plasma glucose levels were maintained at either hypoglycemia (∼ 3 mmol/L) or hyperglycemia (∼ 14 mmol/L). Dopamine was elevated at 100 min (+ 113{\%} above basal levels) and 120 min (+ 117{\%}) in the hypoglycemic condition and at 120 min (+ 121{\%}) in the hyperglycemic condition. In the hyperglycemic post-clamp period, homovanillic acid was decreased below basal levels (approximately - 32{\%}). These results together suggest that short-term blood glucose deviations coupled with acute hyperinsulinemia affect the mesoaccumbens dopamine system.",
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Alterations in blood glucose levels under hyperinsulinemia affect accumbens dopamine. / Bello, Nicholas T.; Hajnal, Andras.

In: Physiology and Behavior, Vol. 88, No. 1-2, 15.06.2006, p. 138-145.

Research output: Contribution to journalArticle

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