Alterations in heparan sulfate in the vessel in response to vascular injury in the mouse.

Neeta Adhikari, Melissa Rusch, Ami Mariash, Qinglu Li, Scott Brian Selleck, Jennifer L. Hall

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Heparan sulfate (HS) is ubiquitous throughout the human body. The backbone of HS is composed of many types of sugars. HS serves as a docking site for a vast array of protein ligands. Recent evidence suggests a unique diversity in HS structure that alters protein binding and protein function. This diversity in HS structure has been overlooked till now. The goal of this study was to determine whether femoral artery wire injury modified HS structure. Femoral artery wire injury was performed in 16-week-old male C57BL6 mice. Transcript levels of a panel of enzymes that regulate HS fine structure, including N-deacetylase-N-sulfotransferases (Ndst) 1 and 2, exostoses (Ext) 1 and 2, C5 epimerase, and 2-O and 6-O sulfotransferases, were quantified with real-time quantitative polymerase chain reaction at 7 and 14 days post injury. All enzymes showed significant alterations in messenger RNA expression in response to injury. Ndst1, the most prevalent isoform, exhibited a 20-fold increase in response to injury. Injury induced significant alterations in fine structure specially increases in N-sulfated disaccharides at 14 days post injury. Vascular injury invokes transcriptional regulation of the enzymes that regulate HS structure, as well as changes in the pattern of HS chains in the vessel wall 14 days post injury. These findings may be important as the foundation of altered growth factor and chemokine binding in the process of vascular remodeling.

Original languageEnglish (US)
Pages (from-to)236-240
Number of pages5
JournalJournal of Cardiovascular Translational Research
Volume1
Issue number3
DOIs
StatePublished - Jan 1 2008

Fingerprint

Heparitin Sulfate
Vascular System Injuries
Wounds and Injuries
Sulfotransferases
Femoral Artery
Enzymes
Exostoses
Racemases and Epimerases
Protein Array Analysis
Disaccharides
Human Body
Chemokines
Protein Binding
Real-Time Polymerase Chain Reaction
Intercellular Signaling Peptides and Proteins
Carrier Proteins
Protein Isoforms
Ligands
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Pharmaceutical Science
  • Cardiology and Cardiovascular Medicine
  • Genetics(clinical)

Cite this

Adhikari, Neeta ; Rusch, Melissa ; Mariash, Ami ; Li, Qinglu ; Selleck, Scott Brian ; Hall, Jennifer L. / Alterations in heparan sulfate in the vessel in response to vascular injury in the mouse. In: Journal of Cardiovascular Translational Research. 2008 ; Vol. 1, No. 3. pp. 236-240.
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Alterations in heparan sulfate in the vessel in response to vascular injury in the mouse. / Adhikari, Neeta; Rusch, Melissa; Mariash, Ami; Li, Qinglu; Selleck, Scott Brian; Hall, Jennifer L.

In: Journal of Cardiovascular Translational Research, Vol. 1, No. 3, 01.01.2008, p. 236-240.

Research output: Contribution to journalArticle

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