Alterations in Plasmodium falciparum genetic structure two years after increased malaria control efforts in western Kenya

Anne M. Vardo-Zalik, Guofa Zhou, Daibin Zhong, Yaw A. Afrane, Andrew K. Githeko, Guiyun Yan

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The impact of malaria intervention measures (insecticide-treated net use and artemisinin combination therapy) on malaria genetics was investigated at two sites in western Kenya: an endemic lowland and an epidemic highland. The genetic structure of the parasite population was assessed by using microsatellites, and the prevalence of drug-resistant mutations was examined by using the polymerase chain reaction-restriction fragment length polymorphism method. Two years after intervention, genetic diversity remained high in both populations. A significant decrease in the prevalence of quintuple mutations conferring resistance to sulfadoxine-pyrimethamine was detected in both populations, but the mutation prevalence at codon 1246 of the Plasmodium falciparum multidrug resistance 1 gene had increased in the highland population. The decrease in sulfadoxine-pyrimethamine-resistant mutants is encouraging, but the increase in P. falciparum multidrug resistance 1 gene mutations is worrisome because these mutations are linked to resistance to other antimalarial drugs. In addition, the high level of genetic diversity observed after intervention suggests transmission is still high in each population.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume88
Issue number1
DOIs
StatePublished - Jan 2013

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Virology
  • Infectious Diseases

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