Alterations in the MA and NC domains modulate phosphoinositide-dependent plasma membrane localization of the Rous sarcoma virus gag protein

Shorena Nadaraia-Hoke, Darrin V. Bann, Timothy L. Lochmann, Nicole Gudleski-O'Regan, Leslie J. Parent

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17 Scopus citations


Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P2 and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P3] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P2 and PI(3,4,5)P3,suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.

Original languageEnglish (US)
Pages (from-to)3609-3615
Number of pages7
JournalJournal of virology
Issue number6
StatePublished - Mar 1 2013


All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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