Abstract
Glycogen and compatible solutes are the major polymeric and soluble carbohydrates in cyanobacteria and function as energy reserves and osmoprotectants, respectively. Glycogen synthase null mutants (glgA-I glgA-II) were constructed in the cyanobacterium Synechococcus sp. strain PCC 7002. Under standard conditions the double mutant produced no glycogen and more soluble sugars. When grown under hypersaline conditions, the glgA-I glgA-II mutant accumulated 1.8-fold more soluble sugars (sucrose and glucosylglycer-(ol/ate)) than WT, and these cells spontaneously excreted soluble sugars into the medium at high levels without the need for additional transporters. An average of 27% more soluble sugars was released from the glgA-I glgA-II mutant than WT by hypo-osmotic shock. Extracellular vesicles budding from the outer membrane were observed by transmission electron microscopy in glgA-I glgA-II cells grown under hypersaline conditions. The glgA-I glgA-II mutant serves as a starting point for developing cell factories for photosynthetic production and excretion of sugars.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 56-67 |
| Number of pages | 12 |
| Journal | Metabolic engineering |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 2013 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002 : Cell factories for soluble sugars. / Xu, Yu; Tiago Guerra, L.; Li, Zhongkui; Ludwig, Marcus; Charles Dismukes, G.; Bryant, Donald Ashley.
In: Metabolic engineering, Vol. 16, No. 1, 01.03.2013, p. 56-67.Research output: Contribution to journal › Article
TY - JOUR
T1 - Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002
T2 - Cell factories for soluble sugars
AU - Xu, Yu
AU - Tiago Guerra, L.
AU - Li, Zhongkui
AU - Ludwig, Marcus
AU - Charles Dismukes, G.
AU - Bryant, Donald Ashley
PY - 2013/3/1
Y1 - 2013/3/1
N2 - Glycogen and compatible solutes are the major polymeric and soluble carbohydrates in cyanobacteria and function as energy reserves and osmoprotectants, respectively. Glycogen synthase null mutants (glgA-I glgA-II) were constructed in the cyanobacterium Synechococcus sp. strain PCC 7002. Under standard conditions the double mutant produced no glycogen and more soluble sugars. When grown under hypersaline conditions, the glgA-I glgA-II mutant accumulated 1.8-fold more soluble sugars (sucrose and glucosylglycer-(ol/ate)) than WT, and these cells spontaneously excreted soluble sugars into the medium at high levels without the need for additional transporters. An average of 27% more soluble sugars was released from the glgA-I glgA-II mutant than WT by hypo-osmotic shock. Extracellular vesicles budding from the outer membrane were observed by transmission electron microscopy in glgA-I glgA-II cells grown under hypersaline conditions. The glgA-I glgA-II mutant serves as a starting point for developing cell factories for photosynthetic production and excretion of sugars.
AB - Glycogen and compatible solutes are the major polymeric and soluble carbohydrates in cyanobacteria and function as energy reserves and osmoprotectants, respectively. Glycogen synthase null mutants (glgA-I glgA-II) were constructed in the cyanobacterium Synechococcus sp. strain PCC 7002. Under standard conditions the double mutant produced no glycogen and more soluble sugars. When grown under hypersaline conditions, the glgA-I glgA-II mutant accumulated 1.8-fold more soluble sugars (sucrose and glucosylglycer-(ol/ate)) than WT, and these cells spontaneously excreted soluble sugars into the medium at high levels without the need for additional transporters. An average of 27% more soluble sugars was released from the glgA-I glgA-II mutant than WT by hypo-osmotic shock. Extracellular vesicles budding from the outer membrane were observed by transmission electron microscopy in glgA-I glgA-II cells grown under hypersaline conditions. The glgA-I glgA-II mutant serves as a starting point for developing cell factories for photosynthetic production and excretion of sugars.
UR - http://www.scopus.com/inward/record.url?scp=84872420422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872420422&partnerID=8YFLogxK
U2 - 10.1016/j.ymben.2012.12.002
DO - 10.1016/j.ymben.2012.12.002
M3 - Article
C2 - 23262095
AN - SCOPUS:84872420422
VL - 16
SP - 56
EP - 67
JO - Metabolic Engineering
JF - Metabolic Engineering
SN - 1096-7176
IS - 1
ER -