Altered Lipid Composition of Secretory Cells Following Exposure to Zinc Can Be Correlated to Changes in Exocytosis

Lin Ren, Masoumeh Dowlatshahi Pour, Per Malmberg, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

A micromolar concentration of zinc has been shown to significantly change the dynamics of exocytosis as well as the vesicle contents in a model cell line, providing direct evidence that zinc regulates neurotransmitter release. To provide insight into how zinc modulates these exocytotic processes, neurotransmitter release and vesicle content were compared with single cell amperometry and intracellular impact vesicle cytometry with a range of zinc concentrations. Additionally, time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of lipid distributions in the cell membrane after zinc treatment correlate to changes in exocytosis. By combining electrochemical techniques and mass spectrometry imaging, we proposed a mechanism by which zinc changes the fusion pore and the rate of neurotransmitter release by changing lipid distributions and results in the modulation of synaptic strength and plasticity.

Original languageEnglish (US)
Pages (from-to)5406-5411
Number of pages6
JournalChemistry - A European Journal
Volume25
Issue number21
DOIs
StatePublished - Apr 11 2019

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Organic Chemistry

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