Nuclei were isolated from rat liver and incubated invitro in two commonly employed RNA transport assays. Released [14C] RNA was isolated and hybridized with filter-bound DNA in the presence of competing cytoplasmic RNA. A significant portion of RNA which was transported to either medium was not represented in cytoplasmic RNA. These results indicate that the restriction of some sequences to the nucleus invivo is not maintained invitro.
All Science Journal Classification (ASJC) codes
- Molecular Biology