AMID mediates adenosine-induced caspase-independent HuH-7 cell apoptosis

Dongqin Yang, Takahiro Yaguchi, Tetsu Nagata, Akinobu Gotoh, Sinisa Dovat, Chunhua Song, Tomoyuki Nishizaki

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Abstract

Background/Aims: The mechanism underlying extracellular adenosine-induced caspase-independent apoptosis in HuH-7 human hepatoma cells is not fully understood. The present study investigated the role for apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death (AMID) in the pathway. Methods: To see the implication of AMID in adenosine-induced HuH-7 cell apoptosis, real-time reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescent cytochemistry, time-laps GFP monitoring, cell cycle analysis, flow cytometry, Western blotting, cell viability assay, and TUNEL staining were carried out. Results: Adenosine upregulated AMID expression in HuH-7 cells, and translocated AMID from the cytosol into the nucleus. Adenosine induced HuH-7 cell apoptosis, and the effect was further enhanced by overexpressing AMID. Adenosine-induced HuH-7 cell apoptosis, alternatively, was inhibited by knocking-down AMID. Conclusion: The results of the present study provide evidence for AMID as a critical factor for adenosine-induced caspase-independent HuH-7 cell apoptosis.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalCellular Physiology and Biochemistry
Volume27
Issue number1
DOIs
StatePublished - 2011

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All Science Journal Classification (ASJC) codes

  • Physiology

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