Amino acid networks in a (β/α)8 barrel enzyme change during catalytic turnover

Jennifer M. Axe, Eric M. Yezdimer, Kathleen F. Orourke, Nicole E. Kerstetter, Wanli You, Chia En A. Chang, David D. Boehr

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Proteins can be viewed as small-world networks of amino acid residues connected through noncovalent interactions. Nuclear magnetic resonance chemical shift covariance analyses were used to identify long-range amino acid networks in the α subunit of tryptophan synthase both for the resting state (in the absence of substrate and product) and for the working state (during catalytic turnover). The amino acid networks observed stretch from the surface of the protein into the active site and are different between the resting and working states. Modification of surface residues on the network alters the structural dynamics of active-site residues over 25 Å away and leads to changes in catalytic rates. These findings demonstrate that amino acid networks, similar to those studied here, are likely important for coordinating structural changes necessary for enzyme function and regulation.

Original languageEnglish (US)
Pages (from-to)6818-6821
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number19
DOIs
StatePublished - May 14 2014

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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    Axe, J. M., Yezdimer, E. M., Orourke, K. F., Kerstetter, N. E., You, W., Chang, C. E. A., & Boehr, D. D. (2014). Amino acid networks in a (β/α)8 barrel enzyme change during catalytic turnover. Journal of the American Chemical Society, 136(19), 6818-6821. https://doi.org/10.1021/ja501602t