Aminoglycoside-Resistant Streptococcus and Enterococcus Species Isolated from Bovine Mammary Secretions

Bhushan M. Jayarao, S. P. Oliver

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A total of 117 isolates representing four Streptococcus species and 20 isolates representing two Enterococcus species from bovine mammary secretions were examined for resistance to streptomycin, kanamycin, and gentamicin. Resistance to streptomycin (85.4%) was most prevalent, followed by kanamycin (19%) and gentamicin (2.2%). Minimum inhibitory concentration of streptomycin for most organisms examined ranged from 16 to 250 µg/ml. For kanamycin, the minimum inhibitory concentration for most organisms was .5 to 62.5 µg/ml. Minimum inhibitory concentration for gentamicin was lower than for kanamycin. Two strains each of Streptococcus uberis and Enterococcus faecalis had minimum inhibitory concentration for streptomycin of >2000 µg/ml, and two strains of E. faecalis had similar minimum inhibitory concentration for kanamycin. The minimum bactericidal concentration for all organisms was one to four times higher than minimum inhibitory concentration. None of the organisms evaluated was found to carry plasmids. Transfer of antibiotic resistance in plasmid-free strains was not achieved by mobilization with the aid of plasmid pAD1, indicating the absence of conjugative transposons. These data suggest that expression of aminoglycoside resistance of Streptococcus and Enterococcus species of bovine origin is likely due to factors other than plasmids or conjugative transposons.

Original languageEnglish (US)
Pages (from-to)991-997
Number of pages7
JournalJournal of dairy science
Volume75
Issue number4
DOIs
StatePublished - Jan 1 1992

Fingerprint

aminoglycosides
Streptococcus
Enterococcus
Microbial Sensitivity Tests
Aminoglycosides
minimum inhibitory concentration
Kanamycin
breasts
kanamycin
Breast
secretion
Streptomycin
streptomycin
Enterococcus faecalis
cattle
plasmids
Plasmids
gentamicin
Gentamicins
organisms

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

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abstract = "A total of 117 isolates representing four Streptococcus species and 20 isolates representing two Enterococcus species from bovine mammary secretions were examined for resistance to streptomycin, kanamycin, and gentamicin. Resistance to streptomycin (85.4{\%}) was most prevalent, followed by kanamycin (19{\%}) and gentamicin (2.2{\%}). Minimum inhibitory concentration of streptomycin for most organisms examined ranged from 16 to 250 µg/ml. For kanamycin, the minimum inhibitory concentration for most organisms was .5 to 62.5 µg/ml. Minimum inhibitory concentration for gentamicin was lower than for kanamycin. Two strains each of Streptococcus uberis and Enterococcus faecalis had minimum inhibitory concentration for streptomycin of >2000 µg/ml, and two strains of E. faecalis had similar minimum inhibitory concentration for kanamycin. The minimum bactericidal concentration for all organisms was one to four times higher than minimum inhibitory concentration. None of the organisms evaluated was found to carry plasmids. Transfer of antibiotic resistance in plasmid-free strains was not achieved by mobilization with the aid of plasmid pAD1, indicating the absence of conjugative transposons. These data suggest that expression of aminoglycoside resistance of Streptococcus and Enterococcus species of bovine origin is likely due to factors other than plasmids or conjugative transposons.",
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Aminoglycoside-Resistant Streptococcus and Enterococcus Species Isolated from Bovine Mammary Secretions. / Jayarao, Bhushan M.; Oliver, S. P.

In: Journal of dairy science, Vol. 75, No. 4, 01.01.1992, p. 991-997.

Research output: Contribution to journalArticle

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AB - A total of 117 isolates representing four Streptococcus species and 20 isolates representing two Enterococcus species from bovine mammary secretions were examined for resistance to streptomycin, kanamycin, and gentamicin. Resistance to streptomycin (85.4%) was most prevalent, followed by kanamycin (19%) and gentamicin (2.2%). Minimum inhibitory concentration of streptomycin for most organisms examined ranged from 16 to 250 µg/ml. For kanamycin, the minimum inhibitory concentration for most organisms was .5 to 62.5 µg/ml. Minimum inhibitory concentration for gentamicin was lower than for kanamycin. Two strains each of Streptococcus uberis and Enterococcus faecalis had minimum inhibitory concentration for streptomycin of >2000 µg/ml, and two strains of E. faecalis had similar minimum inhibitory concentration for kanamycin. The minimum bactericidal concentration for all organisms was one to four times higher than minimum inhibitory concentration. None of the organisms evaluated was found to carry plasmids. Transfer of antibiotic resistance in plasmid-free strains was not achieved by mobilization with the aid of plasmid pAD1, indicating the absence of conjugative transposons. These data suggest that expression of aminoglycoside resistance of Streptococcus and Enterococcus species of bovine origin is likely due to factors other than plasmids or conjugative transposons.

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