Ammonia- and nitrite-oxidizing bacterial communities in a pilot-scale chloraminated drinking water distribution system

John Ragan, G. W. Harrington, D. R. Noguera

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Nitrification in drinking water distribution systems is a common operational problem for many utilities that use chloramines for secondary disinfection. The diversity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the distribution systems of a pilot-scale chloraminated drinking water treatment system was characterized using terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene (ribosomal DNA [rDNA]) cloning and sequencing. For ammonia oxidizers, 16S rDNA-targeted T-RFLP indicated the presence of Nitrosomonas in each of the distribution systems, with a considerably smaller peak attributable to Nitrosospira-like AOB. Sequences of AOB amplification products aligned within the Nitrosomonas oligotropha cluster and were closely related to N. oligotropha and Nitrosomonas ureae. The nitrite-oxidizing communities were comprised primarily of Nitrospira, although Nitrobacter was detected in some samples. These results suggest a possible selection of AOB related to N. oligotropha and N. ureae in chloraminated systems and demonstrate the presence of NOB, indicating a biological mechanism for nitrite loss that contributes to a reduction in nitrite-associated chloramine decay.

Original languageEnglish (US)
Pages (from-to)73-81
Number of pages9
JournalApplied and environmental microbiology
Volume68
Issue number1
DOIs
StatePublished - Jan 15 2002

Fingerprint

water distribution
Nitrites
Ammonia
bacterial communities
Drinking Water
nitrites
nitrite
drinking water
Nitrosomonas oligotropha
ammonia
Nitrosomonas
Bacteria
Nitrosomonas ureae
bacterium
bacteria
Ribosomal DNA
distribution system
Restriction Fragment Length Polymorphisms
ribosomal DNA
restriction fragment length polymorphism

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

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Ammonia- and nitrite-oxidizing bacterial communities in a pilot-scale chloraminated drinking water distribution system. / Ragan, John; Harrington, G. W.; Noguera, D. R.

In: Applied and environmental microbiology, Vol. 68, No. 1, 15.01.2002, p. 73-81.

Research output: Contribution to journalArticle

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