An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits

Satoshi Takahashi, Levente Kapa ́s, Jidong Fang, James M. Krueger

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Abstract

It is hypothesized that tumor necrosis factor (TNF) is an endogenous sleep-promoting substance. In the present experiments we studied the effects of a monoclonal anti-TNF antibody in rats and rabbits. Seven rats and 14 rabbits were implanted with electroencephalographic electrodes, a brain thermistor and an intracerebroventricular guide cannula. The animals were injected with saline, control IgG, and monoclonal hamster anti-murine-TNF antibodies (TNFab) on 3 separate days. Ten μg TNFab suppressed non-rapid-eye-movement sleep (NREMS) in rats. In rabbits, 2.5 μg TNFab did not affect sleep but decreased brain temperature; in contrast, 25 μg TNFab suppressed NREMS without affecting brain temperature. These results are consistent with the hypothesis that endogenous TNF plays an important role in sleep regulation.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalBrain research
Volume690
Issue number2
DOIs
Publication statusPublished - Sep 4 1995

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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