An approach to histone nearest neighbours in extended chromatin

Ross Cameron Hardison, Mary Ellen Eichner, Roger Chalkley

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The primary sequence organization of histones upon the DNA molecule in chromatin has been analyzed by extension of the nucleoprotein at very low ionic strength and crosslinking with a reversible crosslinking reagent, methyl-4-mercaptobutyrimidate. Histones extracted after limited reaction were fractionated into different classes and the composition of the oligomers analyzed after reduction of the crosslinked material. We have found that the following dimers occur at a high frequency: (F3-F2b), (F3-F2a2), and (F2b-F2a2), whereas (F2b-F2a1), (F3-F2a1) and (F3-F3) occur with a lower frequency. F1 appears to polymerize rapidly to largely homogeneous polymers of high molecular weight. These results are analyzed in terms of several models proposed for chromatin structure.

Original languageEnglish (US)
Pages (from-to)1751-1770
Number of pages20
JournalNucleic acids research
Volume2
Issue number10
DOIs
StatePublished - Oct 1 1975

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Histones
Chromatin
Cross-Linking Reagents
Nucleoproteins
Osmolar Concentration
Polymers
Molecular Weight
DNA
methyl 4-mercaptobutyrimidate

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Hardison, Ross Cameron ; Eichner, Mary Ellen ; Chalkley, Roger. / An approach to histone nearest neighbours in extended chromatin. In: Nucleic acids research. 1975 ; Vol. 2, No. 10. pp. 1751-1770.
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An approach to histone nearest neighbours in extended chromatin. / Hardison, Ross Cameron; Eichner, Mary Ellen; Chalkley, Roger.

In: Nucleic acids research, Vol. 2, No. 10, 01.10.1975, p. 1751-1770.

Research output: Contribution to journalArticle

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