An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant

D. C. Bassham, Susannah Gal, A. Da Silva Conceicao, N. V. Raikhel

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The syntaxin family of integral membrane proteins are thought to function as receptors for transport vesicles, with different isoforms of this family localized to various membranes throughout the cell. The yeast Pep12 protein is a syntaxin homologue which may function in the trafficking of vesicles from the trans-Golgi network to the vacuole. We have isolated an Arabidopsis thaliana cDNA by functional complementation of a yeast pep12 mutant. The Arabidopsis cDNA (aPEP12) potentially encodes a 31-kDa protein which is homologous to yeast Pep12 and to other members of the syntaxin family, indicating that this protein may function in the docking or fusion of transport vesicles with the vacuolar membrane in plant cells. Northern blot analysis indicates that the mRNA is expressed in all tissues examined, although at a very low level in leaves. The mRNA is found in all cell types in roots and leaves, as shown by in situ hybridization experiments. The existence of plant homologues of proteins of the syntaxin family indicates that the basic vesicle docking and fusion machinery may be conserved in plants as it is in yeast and mammals.

Original languageEnglish (US)
Pages (from-to)7262-7266
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number16
DOIs
StatePublished - Aug 15 1995

Fingerprint

Qa-SNARE Proteins
Arabidopsis
Yeasts
Transport Vesicles
Complementary DNA
trans-Golgi Network
Plant Proteins
Messenger RNA
Fungal Proteins
Plant Cells
Vacuoles
Northern Blotting
In Situ Hybridization
Mammals
Protein Isoforms
Membrane Proteins
Proteins
Cell Membrane
Membranes

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{7ad60067095d4ca5a90b12dabd4b995e,
title = "An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant",
abstract = "The syntaxin family of integral membrane proteins are thought to function as receptors for transport vesicles, with different isoforms of this family localized to various membranes throughout the cell. The yeast Pep12 protein is a syntaxin homologue which may function in the trafficking of vesicles from the trans-Golgi network to the vacuole. We have isolated an Arabidopsis thaliana cDNA by functional complementation of a yeast pep12 mutant. The Arabidopsis cDNA (aPEP12) potentially encodes a 31-kDa protein which is homologous to yeast Pep12 and to other members of the syntaxin family, indicating that this protein may function in the docking or fusion of transport vesicles with the vacuolar membrane in plant cells. Northern blot analysis indicates that the mRNA is expressed in all tissues examined, although at a very low level in leaves. The mRNA is found in all cell types in roots and leaves, as shown by in situ hybridization experiments. The existence of plant homologues of proteins of the syntaxin family indicates that the basic vesicle docking and fusion machinery may be conserved in plants as it is in yeast and mammals.",
author = "Bassham, {D. C.} and Susannah Gal and {Da Silva Conceicao}, A. and Raikhel, {N. V.}",
year = "1995",
month = "8",
day = "15",
doi = "10.1073/pnas.92.16.7262",
language = "English (US)",
volume = "92",
pages = "7262--7266",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "16",

}

An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant. / Bassham, D. C.; Gal, Susannah; Da Silva Conceicao, A.; Raikhel, N. V.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 16, 15.08.1995, p. 7262-7266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An Arabidopsis syntaxin homologue isolated by functional complementation of a yeast pep12 mutant

AU - Bassham, D. C.

AU - Gal, Susannah

AU - Da Silva Conceicao, A.

AU - Raikhel, N. V.

PY - 1995/8/15

Y1 - 1995/8/15

N2 - The syntaxin family of integral membrane proteins are thought to function as receptors for transport vesicles, with different isoforms of this family localized to various membranes throughout the cell. The yeast Pep12 protein is a syntaxin homologue which may function in the trafficking of vesicles from the trans-Golgi network to the vacuole. We have isolated an Arabidopsis thaliana cDNA by functional complementation of a yeast pep12 mutant. The Arabidopsis cDNA (aPEP12) potentially encodes a 31-kDa protein which is homologous to yeast Pep12 and to other members of the syntaxin family, indicating that this protein may function in the docking or fusion of transport vesicles with the vacuolar membrane in plant cells. Northern blot analysis indicates that the mRNA is expressed in all tissues examined, although at a very low level in leaves. The mRNA is found in all cell types in roots and leaves, as shown by in situ hybridization experiments. The existence of plant homologues of proteins of the syntaxin family indicates that the basic vesicle docking and fusion machinery may be conserved in plants as it is in yeast and mammals.

AB - The syntaxin family of integral membrane proteins are thought to function as receptors for transport vesicles, with different isoforms of this family localized to various membranes throughout the cell. The yeast Pep12 protein is a syntaxin homologue which may function in the trafficking of vesicles from the trans-Golgi network to the vacuole. We have isolated an Arabidopsis thaliana cDNA by functional complementation of a yeast pep12 mutant. The Arabidopsis cDNA (aPEP12) potentially encodes a 31-kDa protein which is homologous to yeast Pep12 and to other members of the syntaxin family, indicating that this protein may function in the docking or fusion of transport vesicles with the vacuolar membrane in plant cells. Northern blot analysis indicates that the mRNA is expressed in all tissues examined, although at a very low level in leaves. The mRNA is found in all cell types in roots and leaves, as shown by in situ hybridization experiments. The existence of plant homologues of proteins of the syntaxin family indicates that the basic vesicle docking and fusion machinery may be conserved in plants as it is in yeast and mammals.

UR - http://www.scopus.com/inward/record.url?scp=0029119236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029119236&partnerID=8YFLogxK

U2 - 10.1073/pnas.92.16.7262

DO - 10.1073/pnas.92.16.7262

M3 - Article

C2 - 7638178

AN - SCOPUS:0029119236

VL - 92

SP - 7262

EP - 7266

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 16

ER -