An astrocytic binding site for neuronal Thy-1 and its effect on neurite outgrowth

Evan B. Dreyer, Dana Leifer, John E. Heng, Jane E. McConnell, Madhu Gorla, Leonard A. Levin, Colin Barnstable, Stuart A. Lipton

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Thy-1, a member of the immunoglobulin superfamily, is one of the most abundant glycoproteins on mammalian neurons. Nevertheless, its role in the peripheral or central nervous system is poorly understood. Certain monoclonal antibodies to Thy-1 promote neurite outgrowth by rodent central nervous system neurons in vitro, suggesting that Thy-1 functions, in part, by modulating neurite outgrowth. We describe a binding site for Thy-1 on astrocytes. This Thy-1-binding protein has been characterized by immunofluorescence with specific anti-idiotype monoclonal antibodies and by three competitive binding assays using (i) anti-idiotype antibodies, (ii) purified Thy-1, and (iii) Thy-1-transfected cells. The Thy-1-binding protein may participate in axonal or dendritic development in the nervous system.

Original languageEnglish (US)
Pages (from-to)11195-11199
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number24
DOIs
StatePublished - Nov 21 1995

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Anti-Idiotypic Antibodies
Carrier Proteins
Central Nervous System
Binding Sites
Monoclonal Antibodies
Neurons
Competitive Binding
Peripheral Nervous System
Astrocytes
Nervous System
Fluorescent Antibody Technique
Immunoglobulins
Rodentia
Glycoproteins
Neuronal Outgrowth
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • General

Cite this

Dreyer, E. B., Leifer, D., Heng, J. E., McConnell, J. E., Gorla, M., Levin, L. A., ... Lipton, S. A. (1995). An astrocytic binding site for neuronal Thy-1 and its effect on neurite outgrowth. Proceedings of the National Academy of Sciences of the United States of America, 92(24), 11195-11199. https://doi.org/10.1073/pnas.92.24.11195
Dreyer, Evan B. ; Leifer, Dana ; Heng, John E. ; McConnell, Jane E. ; Gorla, Madhu ; Levin, Leonard A. ; Barnstable, Colin ; Lipton, Stuart A. / An astrocytic binding site for neuronal Thy-1 and its effect on neurite outgrowth. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 24. pp. 11195-11199.
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An astrocytic binding site for neuronal Thy-1 and its effect on neurite outgrowth. / Dreyer, Evan B.; Leifer, Dana; Heng, John E.; McConnell, Jane E.; Gorla, Madhu; Levin, Leonard A.; Barnstable, Colin; Lipton, Stuart A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 24, 21.11.1995, p. 11195-11199.

Research output: Contribution to journalArticle

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AU - Dreyer, Evan B.

AU - Leifer, Dana

AU - Heng, John E.

AU - McConnell, Jane E.

AU - Gorla, Madhu

AU - Levin, Leonard A.

AU - Barnstable, Colin

AU - Lipton, Stuart A.

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