An astrocytic binding site for neuronal Thy-1 and its effect on neurite outgrowth

Evan B. Dreyer, Dana Leifer, John E. Heng, Jane E. McConnell, Madhu Gorla, Leonard A. Levin, Colin Barnstable, Stuart A. Lipton

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Thy-1, a member of the immunoglobulin superfamily, is one of the most abundant glycoproteins on mammalian neurons. Nevertheless, its role in the peripheral or central nervous system is poorly understood. Certain monoclonal antibodies to Thy-1 promote neurite outgrowth by rodent central nervous system neurons in vitro, suggesting that Thy-1 functions, in part, by modulating neurite outgrowth. We describe a binding site for Thy-1 on astrocytes. This Thy-1-binding protein has been characterized by immunofluorescence with specific anti-idiotype monoclonal antibodies and by three competitive binding assays using (i) anti-idiotype antibodies, (ii) purified Thy-1, and (iii) Thy-1-transfected cells. The Thy-1-binding protein may participate in axonal or dendritic development in the nervous system.

Original languageEnglish (US)
Pages (from-to)11195-11199
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number24
DOIs
StatePublished - Nov 21 1995

All Science Journal Classification (ASJC) codes

  • General

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