An atypical heterotrimeric G-protein β-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana

David Chakravorty, Yuri Trusov, Wei Zhang, Biswa R. Acharya, Michael B. Sheahan, David W. McCurdy, Sarah Mary Assmann, José Ramõn Botella

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Summary Currently, there are strong inconsistencies in our knowledge of plant heterotrimeric G-proteins that suggest the existence of additional members of the family. We have identified a new Arabidopsis G-protein β-subunit (AGG3) that modulates morphological development and ABA-regulation of stomatal aperture. AGG3 strongly interacts with the Arabidopsis G-protein β-subunit in vivo and in vitro. Most importantly, AGG3-deficient mutants account for all but one of the 'orphan' phenotypes previously unexplained by the two known β-subunits in Arabidopsis. AGG3 has unique characteristics never before observed in plant or animal systems, such as its size (more than twice that of canonical β-subunits) and the presence of a C-terminal Cys-rich domain. AGG3 thus represent a novel class of G-protein β-subunits, widely spread throughout the plant kingdom but not present in animals. Homologues of AGG3 in rice have been identified as important quantitative trait loci for grain size and yield, but due to the atypical nature of the proteins their identity as G-protein subunits was thus far unknown. Our work demonstrates a similar trend in seeds of Arabidopsis agg3 mutants, and implicates G-proteins in such a crucial agronomic trait. The discovery of this highly atypical subunit reinforces the emerging notion that plant and animal G-proteins have distinct as well as shared evolutionary pathways.

Original languageEnglish (US)
Pages (from-to)840-851
Number of pages12
JournalPlant Journal
Volume67
Issue number5
DOIs
StatePublished - Sep 1 2011

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Heterotrimeric GTP-Binding Proteins
potassium channels
Protein Subunits
guard cells
protein subunits
G-proteins
GTP-Binding Proteins
Arabidopsis
Arabidopsis thaliana
Arabidopsis Proteins
Quantitative Trait Loci
mutants
animals
Seeds
agronomic traits
quantitative trait loci
Phenotype
phenotype
rice
seeds

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Chakravorty, David ; Trusov, Yuri ; Zhang, Wei ; Acharya, Biswa R. ; Sheahan, Michael B. ; McCurdy, David W. ; Assmann, Sarah Mary ; Botella, José Ramõn. / An atypical heterotrimeric G-protein β-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana. In: Plant Journal. 2011 ; Vol. 67, No. 5. pp. 840-851.
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An atypical heterotrimeric G-protein β-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana. / Chakravorty, David; Trusov, Yuri; Zhang, Wei; Acharya, Biswa R.; Sheahan, Michael B.; McCurdy, David W.; Assmann, Sarah Mary; Botella, José Ramõn.

In: Plant Journal, Vol. 67, No. 5, 01.09.2011, p. 840-851.

Research output: Contribution to journalArticle

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