An efficient method for measuring plasma volume using indocyanine green dye

Sixtus Aguree, Alison Diane Gernand

Research output: Contribution to journalArticle

Abstract

Plasma volume (PV) can be an important marker of health status and may affect the interpretation of plasma biomarkers, but is rarely measured due to the complexity and time required. Indocyanine green (ICG) is a water-soluble tricarbocyanine dye with a circulatory half-life of 2–3 min, allowing for quick clearance and repeated use. It is used extensively in medical diagnostic tests including ophthalmologic imaging, liver function, and cardiac output, particularly in critical care. ICG has been validated for measuring PV in humans, however previous work has provided minimal published details or has focused on a single aspect of the method. We aimed to develop a detailed, optimal protocol for the use of ICG to measure PV in women of reproductive age. We combined best practices from other studies and optimized the protocol for efficiency. • This method reduces the time from blood collection to PV determination to ˜2 h and the amount of plasma required to estimate PV to 2.5 mL (1.5 mL before ICG injection and 1.0 mL post-injection). • Participant inconvenience is reduced by inserting an intravenous (IV) catheter in only one arm, not both arms. • Five post-injection plasma samples (2–5 min after ICG bolus) are enough to accurately develop the decay curve for plasma ICG concentration and estimate PV by extrapolation.

Original languageEnglish (US)
Pages (from-to)1072-1083
Number of pages12
JournalMethodsX
Volume6
DOIs
StatePublished - Jan 1 2019

Fingerprint

Indocyanine Green
Plasma Volume
Coloring Agents
Plasmas
Injections
Critical Care
Practice Guidelines
Routine Diagnostic Tests
Cardiac Output
Health Status
Half-Life
Catheters
Biomarkers
Extrapolation
Water
Liver
Blood
Health
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

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An efficient method for measuring plasma volume using indocyanine green dye. / Aguree, Sixtus; Gernand, Alison Diane.

In: MethodsX, Vol. 6, 01.01.2019, p. 1072-1083.

Research output: Contribution to journalArticle

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