Un protocolo eficiente para el cultivo de meristemas híbridos de sorgo

Translated title of the contribution: An efficient protocol for culturing meristems of sorghum hybrids

B. Sadia, P. C. Josekutty, Shobha Devi Potlakayala, P. Patel, S. Goldman, Sairam V. Rudrabhatla

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium supplemented with 3 mg/L BAP and 1 mg/L TDZ was the most effective hormone combination for multiple shoot production. Regenerated shoots were rooted on MS basal medium. Once rooted, plants were transferred to the greenhouse, where 80% survived to reach maturity.

Original languageSpanish
Pages (from-to)177-181
Number of pages5
JournalPhyton
Volume79
StatePublished - Dec 1 2010

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Sorghum
Meristem
Greenhouses
Sorghum (Poaceae)
meristems
Hormones
shoots
Genotype
genotype
shoot meristems
Bony Callus
micropropagation
Sorghum bicolor
growth regulators
rapid methods
genetic improvement
Regeneration
callus
hormones
greenhouses

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Plant Science

Cite this

Sadia, B. ; Josekutty, P. C. ; Potlakayala, Shobha Devi ; Patel, P. ; Goldman, S. ; Rudrabhatla, Sairam V. / Un protocolo eficiente para el cultivo de meristemas híbridos de sorgo. In: Phyton. 2010 ; Vol. 79. pp. 177-181.
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Sadia, B, Josekutty, PC, Potlakayala, SD, Patel, P, Goldman, S & Rudrabhatla, SV 2010, 'Un protocolo eficiente para el cultivo de meristemas híbridos de sorgo', Phyton, vol. 79, pp. 177-181.

Un protocolo eficiente para el cultivo de meristemas híbridos de sorgo. / Sadia, B.; Josekutty, P. C.; Potlakayala, Shobha Devi; Patel, P.; Goldman, S.; Rudrabhatla, Sairam V.

In: Phyton, Vol. 79, 01.12.2010, p. 177-181.

Research output: Contribution to journalArticle

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AU - Josekutty, P. C.

AU - Potlakayala, Shobha Devi

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AU - Goldman, S.

AU - Rudrabhatla, Sairam V.

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