Abstract
Bloodstream infections are major contributing factors of morbidity and mortality among children. Precise and timely identification of causative agents can improve the clinical management and outcome of the infection, potentially saving lives. Electrochemical biosensors previously described by Gao et al. (2017) have the potential to deliver greater speed and discrimination. However, to date there are no data that determine whether the age of the host would cause bacteria to demonstrate different growth characteristics, or whether pediatric samples would behave differently using this electrochemical biosensor. The importance of this knowledge gap is clear: the preclinical testing phase of this line of research is limited by the relative lack of pediatric healthy blood volunteers to complete this work. Therefore, in this study we have applied this novel technology to diagnose bacteria spiked into pediatric blood and compared directly with adult blood samples. Only 180 µL of blood was utilized from both adult and pediatric volunteers and inoculated with Escherichia coli 67, and the signals generated at different time points were compared. We were able to demonstrate that the signals generated by adult and pediatric blood were not significantly different with this detection technology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 616-625 |
| Number of pages | 10 |
| Journal | SLAS Technology |
| Volume | 22 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1 2017 |
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All Science Journal Classification (ASJC) codes
- Computer Science Applications
- Medical Laboratory Technology
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An Electrochemical Biosensor for Rapid Detection of Pediatric Bloodstream Infections. / Kurundu Hewage, Eranda M.K.; Spear, Debbie; Umstead, Todd M.; Hu, Sanmei; Wang, Ming; Wong, Pak Kin; Chroneos, Zissis; Halstead, E. Scott; Thomas, Neal.
In: SLAS Technology, Vol. 22, No. 6, 01.12.2017, p. 616-625.Research output: Contribution to journal › Article
TY - JOUR
T1 - An Electrochemical Biosensor for Rapid Detection of Pediatric Bloodstream Infections
AU - Kurundu Hewage, Eranda M.K.
AU - Spear, Debbie
AU - Umstead, Todd M.
AU - Hu, Sanmei
AU - Wang, Ming
AU - Wong, Pak Kin
AU - Chroneos, Zissis
AU - Halstead, E. Scott
AU - Thomas, Neal
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Bloodstream infections are major contributing factors of morbidity and mortality among children. Precise and timely identification of causative agents can improve the clinical management and outcome of the infection, potentially saving lives. Electrochemical biosensors previously described by Gao et al. (2017) have the potential to deliver greater speed and discrimination. However, to date there are no data that determine whether the age of the host would cause bacteria to demonstrate different growth characteristics, or whether pediatric samples would behave differently using this electrochemical biosensor. The importance of this knowledge gap is clear: the preclinical testing phase of this line of research is limited by the relative lack of pediatric healthy blood volunteers to complete this work. Therefore, in this study we have applied this novel technology to diagnose bacteria spiked into pediatric blood and compared directly with adult blood samples. Only 180 µL of blood was utilized from both adult and pediatric volunteers and inoculated with Escherichia coli 67, and the signals generated at different time points were compared. We were able to demonstrate that the signals generated by adult and pediatric blood were not significantly different with this detection technology.
AB - Bloodstream infections are major contributing factors of morbidity and mortality among children. Precise and timely identification of causative agents can improve the clinical management and outcome of the infection, potentially saving lives. Electrochemical biosensors previously described by Gao et al. (2017) have the potential to deliver greater speed and discrimination. However, to date there are no data that determine whether the age of the host would cause bacteria to demonstrate different growth characteristics, or whether pediatric samples would behave differently using this electrochemical biosensor. The importance of this knowledge gap is clear: the preclinical testing phase of this line of research is limited by the relative lack of pediatric healthy blood volunteers to complete this work. Therefore, in this study we have applied this novel technology to diagnose bacteria spiked into pediatric blood and compared directly with adult blood samples. Only 180 µL of blood was utilized from both adult and pediatric volunteers and inoculated with Escherichia coli 67, and the signals generated at different time points were compared. We were able to demonstrate that the signals generated by adult and pediatric blood were not significantly different with this detection technology.
UR - http://www.scopus.com/inward/record.url?scp=85034736784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034736784&partnerID=8YFLogxK
U2 - 10.1177/2472630317727704
DO - 10.1177/2472630317727704
M3 - Article
C2 - 28850805
AN - SCOPUS:85034736784
VL - 22
SP - 616
EP - 625
JO - SLAS Technology
JF - SLAS Technology
SN - 2472-6303
IS - 6
ER -