An electrokinetic microdevice for isolation and quantification of circulating cell-free DNA from physiological samples

Ariana Lamanda, Yi Lu, Navrose Gill, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This study reports a hybrid electrokinetic microdevice for rapid concentration and detection of circulating cell-free (cf)DNA. Rapid molecular analysis of cfDNA has the potential to change the current practice of medicine, such as in cancer diagnostics and in monitoring the efficacy of cancer treatments. With a combination of AC electrothermal flow and dielectrophoresis, the hybrid electrokinetic microdevice efficiently concentrates cfDNA from blood plasma and other physiological fluids. In this design, the long-range AC electrothermal flow, which is effective in conductive fluids, drives the cfDNA towards the center of the electrode where dielectrophoretic trapping of the cfDNA occurs. Once the cfDNA is collected at the electrode, the concentration in the blood sample can be quantified by fluorescence analysis with an intercalating dye that binds specifically to double-stranded DNA. The effects of the electrokinetic parameters were elucidated to optimize the design of the device. The device was demonstrated to separate high molecular weight DNA from low molecular weight DNA. Quantitative detection of clinically relevant concentrations of cfDNA was achieved in 10 minutes.

Original languageEnglish (US)
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages544-547
Number of pages4
ISBN (Electronic)9781479989553
DOIs
StatePublished - Aug 5 2015
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015Jun 25 2015

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period6/21/156/25/15

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'An electrokinetic microdevice for isolation and quantification of circulating cell-free DNA from physiological samples'. Together they form a unique fingerprint.

  • Cite this

    Lamanda, A., Lu, Y., Gill, N., & Wong, P. K. (2015). An electrokinetic microdevice for isolation and quantification of circulating cell-free DNA from physiological samples. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 544-547). [7180981] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7180981