An electrophoretic and immunochemical characterization of human surfactant-associated proteins

David S. Phelps, H. William Taeusch, Bradley benson, Samuel Hawgood

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Abstract

We have prepared an antiserum against a serum-free extract of alveolar proteinosis lavage that recognizes the same proteins as an antiserum to human surfactant. Using one and two-dimensional gel electrophoresis, protein blotting and immunostaining we have found proteins with Mr of approx. 35 and 60 kDa to be present in every source of human surfactant we have examined. These proteins are immunologically related to those found in the lavage from alveolar proteinosis patients, have the same electrophoretic characteristics and are not found in serum. The 35 kDa protein is a group of at least eight isoforms ranging in relative molecular mass Mr from 32 to 36 kDa with isoelectric points between 4.8 and 5.5. Neuraminidase digestion studies have shown that at least part of this charge heterogeneity may be due to sialic acid residues. The less abundant form, with a Mr of about 60 kDa is also a sialoglycoprotein with similar isoelectric points.

Original languageEnglish (US)
Pages (from-to)226-238
Number of pages13
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume791
Issue number2
DOIs
Publication statusPublished - Dec 7 1984

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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