An electrophoretic and immunochemical characterization of human surfactant-associated proteins

David S. Phelps, H. William Taeusch, Bradley benson, Samuel Hawgood

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

We have prepared an antiserum against a serum-free extract of alveolar proteinosis lavage that recognizes the same proteins as an antiserum to human surfactant. Using one and two-dimensional gel electrophoresis, protein blotting and immunostaining we have found proteins with Mr of approx. 35 and 60 kDa to be present in every source of human surfactant we have examined. These proteins are immunologically related to those found in the lavage from alveolar proteinosis patients, have the same electrophoretic characteristics and are not found in serum. The 35 kDa protein is a group of at least eight isoforms ranging in relative molecular mass Mr from 32 to 36 kDa with isoelectric points between 4.8 and 5.5. Neuraminidase digestion studies have shown that at least part of this charge heterogeneity may be due to sialic acid residues. The less abundant form, with a Mr of about 60 kDa is also a sialoglycoprotein with similar isoelectric points.

Original languageEnglish (US)
Pages (from-to)226-238
Number of pages13
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume791
Issue number2
DOIs
StatePublished - Dec 7 1984

Fingerprint

Surface-Active Agents
Therapeutic Irrigation
Isoelectric Point
Proteins
Immune Sera
Sialoglycoproteins
Electrophoresis, Gel, Two-Dimensional
Neuraminidase
Molecular mass
N-Acetylneuraminic Acid
Electrophoresis
Serum
Digestion
Protein Isoforms
Gels

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

@article{cec91339d1f44c67a5e13470d4fc1203,
title = "An electrophoretic and immunochemical characterization of human surfactant-associated proteins",
abstract = "We have prepared an antiserum against a serum-free extract of alveolar proteinosis lavage that recognizes the same proteins as an antiserum to human surfactant. Using one and two-dimensional gel electrophoresis, protein blotting and immunostaining we have found proteins with Mr of approx. 35 and 60 kDa to be present in every source of human surfactant we have examined. These proteins are immunologically related to those found in the lavage from alveolar proteinosis patients, have the same electrophoretic characteristics and are not found in serum. The 35 kDa protein is a group of at least eight isoforms ranging in relative molecular mass Mr from 32 to 36 kDa with isoelectric points between 4.8 and 5.5. Neuraminidase digestion studies have shown that at least part of this charge heterogeneity may be due to sialic acid residues. The less abundant form, with a Mr of about 60 kDa is also a sialoglycoprotein with similar isoelectric points.",
author = "Phelps, {David S.} and Taeusch, {H. William} and Bradley benson and Samuel Hawgood",
year = "1984",
month = "12",
day = "7",
doi = "10.1016/0167-4838(84)90013-X",
language = "English (US)",
volume = "791",
pages = "226--238",
journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",
issn = "1570-9639",
publisher = "Elsevier",
number = "2",

}

An electrophoretic and immunochemical characterization of human surfactant-associated proteins. / Phelps, David S.; Taeusch, H. William; benson, Bradley; Hawgood, Samuel.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 791, No. 2, 07.12.1984, p. 226-238.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An electrophoretic and immunochemical characterization of human surfactant-associated proteins

AU - Phelps, David S.

AU - Taeusch, H. William

AU - benson, Bradley

AU - Hawgood, Samuel

PY - 1984/12/7

Y1 - 1984/12/7

N2 - We have prepared an antiserum against a serum-free extract of alveolar proteinosis lavage that recognizes the same proteins as an antiserum to human surfactant. Using one and two-dimensional gel electrophoresis, protein blotting and immunostaining we have found proteins with Mr of approx. 35 and 60 kDa to be present in every source of human surfactant we have examined. These proteins are immunologically related to those found in the lavage from alveolar proteinosis patients, have the same electrophoretic characteristics and are not found in serum. The 35 kDa protein is a group of at least eight isoforms ranging in relative molecular mass Mr from 32 to 36 kDa with isoelectric points between 4.8 and 5.5. Neuraminidase digestion studies have shown that at least part of this charge heterogeneity may be due to sialic acid residues. The less abundant form, with a Mr of about 60 kDa is also a sialoglycoprotein with similar isoelectric points.

AB - We have prepared an antiserum against a serum-free extract of alveolar proteinosis lavage that recognizes the same proteins as an antiserum to human surfactant. Using one and two-dimensional gel electrophoresis, protein blotting and immunostaining we have found proteins with Mr of approx. 35 and 60 kDa to be present in every source of human surfactant we have examined. These proteins are immunologically related to those found in the lavage from alveolar proteinosis patients, have the same electrophoretic characteristics and are not found in serum. The 35 kDa protein is a group of at least eight isoforms ranging in relative molecular mass Mr from 32 to 36 kDa with isoelectric points between 4.8 and 5.5. Neuraminidase digestion studies have shown that at least part of this charge heterogeneity may be due to sialic acid residues. The less abundant form, with a Mr of about 60 kDa is also a sialoglycoprotein with similar isoelectric points.

UR - http://www.scopus.com/inward/record.url?scp=0021736917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021736917&partnerID=8YFLogxK

U2 - 10.1016/0167-4838(84)90013-X

DO - 10.1016/0167-4838(84)90013-X

M3 - Article

C2 - 6439249

AN - SCOPUS:0021736917

VL - 791

SP - 226

EP - 238

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

SN - 1570-9639

IS - 2

ER -