An enhanced discriminatory pulsed-field gel electrophoresis scheme for subtyping Salmonella serotypes heidelberg, Kentucky, SaintPaul, and Hadar

Meili Xi, J. I.E. Zheng, Shaohua Zhao, Eric W. Brown, Jianghong Meng

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Conventional pulsed-field gel electrophoresis (PFGE) protocols, used extensively as a successful approach for subtyping many salmonellae, may be inadequate for discriminating strains sharing levels of homogeneity within the same serotype. Four additional restriction enzymes (SpeI, PacI, SfiI, and NotI), in addition to XbaI and BlnI, were used in PFGE typing of 33 Salmonella Heidelberg, 27 Salmonella Kentucky, 27 Salmonella SaintPaul, and 27 Salmonella Hadar isolates that were recovered from poultry and porcine retail meats from different states of the United States. A dendrogram derived from the combined analysis of six enzymes was highly discriminatory with a Simpson index of diversity value of over 0.950. The ratio of nodes to isolates was more than 0.75 with an average of fewer than three isolates in each polytomy for all four serotypes. Two three-enzyme combinations, SpeI/NotI/SfiI for Salmonella Heidelberg and Salmonella Hadar, and SpeI/BlnI/SfiI for Salmonella Kentucky and Salmonella SaintPaul, were found to have comparable discriminatory abilities of differentiating isolates of these Salmonella serotypes with the six-enzyme combination. The enhanced discriminatory PFGE-based subtyping scheme can be used effectively for the differentiation of strains of the four Salmonella serotypes. The findings also highlight PFGE analysis as a continued essential and informative subtyping method for source tracking and outbreak investigations of these and other Salmonella pathogens.

Original languageEnglish (US)
Pages (from-to)2067-2072
Number of pages6
JournalJournal of Food Protection
Volume71
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
Salmonella
Salmonella Saintpaul
serotypes
Salmonella Kentucky
Salmonella Hadar
Salmonella Heidelberg
enzymes
outbreak investigation
poultry
meat
Enzymes
swine
Serogroup
pathogens
Poultry
Meat
Disease Outbreaks
Swine

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Cite this

Xi, Meili ; Zheng, J. I.E. ; Zhao, Shaohua ; Brown, Eric W. ; Meng, Jianghong. / An enhanced discriminatory pulsed-field gel electrophoresis scheme for subtyping Salmonella serotypes heidelberg, Kentucky, SaintPaul, and Hadar. In: Journal of Food Protection. 2008 ; Vol. 71, No. 10. pp. 2067-2072.
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An enhanced discriminatory pulsed-field gel electrophoresis scheme for subtyping Salmonella serotypes heidelberg, Kentucky, SaintPaul, and Hadar. / Xi, Meili; Zheng, J. I.E.; Zhao, Shaohua; Brown, Eric W.; Meng, Jianghong.

In: Journal of Food Protection, Vol. 71, No. 10, 10.2008, p. 2067-2072.

Research output: Contribution to journalArticle

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