An ephemeral sexual population of Phytophthora infestans in the Northeastern United States and Canada

Giovanna Danies, Kevin Myers, María F. Mideros, Silvia Restrepo, Frank N. Martin, David E.L. Cooke, Christine D. Smart, Jean B. Ristaino, Abby J. Seaman, Beth Krueger Gugino, Niklaus J. Grünwald, William E. Fry

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Phytophthora infestans, the causal agent of late blight disease, has been reported in North America since the mid-nineteenth century. In the United States the lack of or very limited sexual reproduction has resulted in largely clonal populations of P. infestans. In 2010 and 2011, but not in 2012 or 2013, 20 rare and diverse genotypes of P. infestans were detected in a region that centered around central New York State. The ratio of A1 to A2 mating types among these genotypes was close to the 50∶50 ratio expected for sexual recombination. These genotypes were diverse at the glucose-6-phosphate isomerase locus, differed in their microsatellite profiles, showed different banding patterns in a restriction fragment length polymorphism assay using a moderately repetitive and highly polymorphic probe (RG57), were polymorphic for four different nuclear genes and differed in their sensitivity to the systemic fungicide mefenoxam. The null hypothesis of linkage equilibrium was not rejected, which suggests the population could be sexual. These new genotypes were monomorphic in their mitochondrial haplotype that was the same as US-22. Through parentage exclusion testing using microsatellite data and sequences of four nuclear genes, recent dominant lineages US-8, US-11, US-23, and US-24 were excluded as possible parents for these genotypes. Further analyses indicated that US-22 could not be eliminated as a possible parent for 14 of the 20 genotypes. We conclude that US-22 could be a parent of some, but not all, of the new genotypes found in 2010 and 2011. There were at least two other parents for this population and the genotypic characteristics of the other parents were identified.

Original languageEnglish (US)
Pages (from-to)e116354
JournalPloS one
Volume9
Issue number12
DOIs
StatePublished - Jan 1 2014

Fingerprint

Phytophthora infestans
New England
Northeastern United States
Microsatellite Repeats
Canada
Genes
Genotype
Glucose-6-Phosphate Isomerase
Fungicides
genotype
Polymorphism
Population
Assays
Testing
glucose-6-phosphate isomerase
microsatellite repeats
Dominant Genes
dominant genes
parentage
Population Characteristics

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Danies, G., Myers, K., Mideros, M. F., Restrepo, S., Martin, F. N., Cooke, D. E. L., ... Fry, W. E. (2014). An ephemeral sexual population of Phytophthora infestans in the Northeastern United States and Canada. PloS one, 9(12), e116354. https://doi.org/10.1371/journal.pone.0116354
Danies, Giovanna ; Myers, Kevin ; Mideros, María F. ; Restrepo, Silvia ; Martin, Frank N. ; Cooke, David E.L. ; Smart, Christine D. ; Ristaino, Jean B. ; Seaman, Abby J. ; Gugino, Beth Krueger ; Grünwald, Niklaus J. ; Fry, William E. / An ephemeral sexual population of Phytophthora infestans in the Northeastern United States and Canada. In: PloS one. 2014 ; Vol. 9, No. 12. pp. e116354.
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Danies, G, Myers, K, Mideros, MF, Restrepo, S, Martin, FN, Cooke, DEL, Smart, CD, Ristaino, JB, Seaman, AJ, Gugino, BK, Grünwald, NJ & Fry, WE 2014, 'An ephemeral sexual population of Phytophthora infestans in the Northeastern United States and Canada', PloS one, vol. 9, no. 12, pp. e116354. https://doi.org/10.1371/journal.pone.0116354

An ephemeral sexual population of Phytophthora infestans in the Northeastern United States and Canada. / Danies, Giovanna; Myers, Kevin; Mideros, María F.; Restrepo, Silvia; Martin, Frank N.; Cooke, David E.L.; Smart, Christine D.; Ristaino, Jean B.; Seaman, Abby J.; Gugino, Beth Krueger; Grünwald, Niklaus J.; Fry, William E.

In: PloS one, Vol. 9, No. 12, 01.01.2014, p. e116354.

Research output: Contribution to journalArticle

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AU - Danies, Giovanna

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AU - Mideros, María F.

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AU - Cooke, David E.L.

AU - Smart, Christine D.

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AU - Fry, William E.

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