An Epstein-Barr virus transforming protein associates with vimentin in lymphocytes.

D. Liebowitz, R. Kopan, E. Fuchs, Jeffery Sample, E. Kieff

Research output: Contribution to journalArticle

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Abstract

The Epstein-Barr virus (EBV) latent infection membrane protein (LMP) is likely to be an important mediator of EBV-induced cell proliferation, since it is one of the few proteins encoded by the virus in latent infection and since production of this protein in Rat-1 cells results in their conversion to a fully transformed phenotype. LMP was previously noted to localize to patches at the cell periphery. In this paper we examine the basis of LMP patching in EBV-infected, transformed lymphocytes. Our data indicate that LMP is associated with the cytoskeletal protein vimentin. Although LMP is fully soluble in isotonic Triton X-100 buffer, only 50% of it is extracted from cells in this solution. The rest remains bound to the cytoskeleton. LMP undergoes phosphorylation, and phosphorylated LMP is preferentially associated with the cytoskeleton. As judged by both immunofluorescence and immunoelectron microscopy, the vimentin network in EBV-transformed lymphocytes or EBV-infected Burkitt tumor lymphocytes is abnormal. Vimentin and LMP often colocalize in a single patch near the plasma membrane. In response to Colcemid treatment of EBV-infected cells, vimentin reorganizes into perinuclear rings, as it does in uninfected cells. LMP is associated with these perinuclear rings. Vimentin (or a vimentin-associated protein) may be a transducer of an LMP transmembrane effect in lymphoproliferation.

Original languageEnglish (US)
Pages (from-to)2299-2308
Number of pages10
JournalMolecular and cellular biology
Volume7
Issue number7
DOIs
StatePublished - Jan 1 1987

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Vimentin
Human Herpesvirus 4
Membrane Proteins
Lymphocytes
Infection
Proteins
Cytoskeleton
Demecolcine
Epstein-Barr Virus Infections
Cytoskeletal Proteins
Burkitt Lymphoma
Immunoelectron Microscopy
Octoxynol
Transducers
Fluorescence Microscopy
Buffers
Phosphorylation
Cell Proliferation
Cell Membrane
Viruses

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Liebowitz, D. ; Kopan, R. ; Fuchs, E. ; Sample, Jeffery ; Kieff, E. / An Epstein-Barr virus transforming protein associates with vimentin in lymphocytes. In: Molecular and cellular biology. 1987 ; Vol. 7, No. 7. pp. 2299-2308.
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An Epstein-Barr virus transforming protein associates with vimentin in lymphocytes. / Liebowitz, D.; Kopan, R.; Fuchs, E.; Sample, Jeffery; Kieff, E.

In: Molecular and cellular biology, Vol. 7, No. 7, 01.01.1987, p. 2299-2308.

Research output: Contribution to journalArticle

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