An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures

D. H. Bremner, W. J. Magill, Nigel Deighton, E. E. Benson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to non-embryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalPhyton - Annales Rei Botanicae
Volume37
Issue number3
StatePublished - Jan 7 1997

All Science Journal Classification (ASJC) codes

  • Global and Planetary Change
  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Fingerprint Dive into the research topics of 'An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures'. Together they form a unique fingerprint.

  • Cite this