34 Citations (Scopus)

Abstract

We have established several HLA-A2.1-transgenic rabbit lines to provide a host to study CD8+ T cell responses during virus infections. HLA-A2.1 protein expression was detected on cell surfaces within various organ tissues. Continuous cultured cells from these transgenic rabbits were capable of presenting both endogenous and exogenous HLA-A2.1-restricted epitopes to an HLA-A2.1-restricted epitope-specific CTL clone. A DNA vaccine containing an HLA-A2.1-restricted human papillomavirus type 16 E7 epitope (amino acid residues 82-90) stimulated epitope-specific CTLs in both PBLs and spleen cells of transgenic rabbits. In addition, vaccinated transgenic rabbits were protected against infection with a mutant cottontail rabbit papillomavirus DNA containing an embedded human papillomavirus type 16 E7/82-90 epitope. Complete protection was achieved using a multivalent epitope DNA vaccine based on epitope selection from cottontail rabbit papillomavirus E1 using MHC class I epitope prediction software. HLA-A2.1-transgenic rabbits will be an important preclinical animal model system to study virus-host interactions and to assess specific targets for immunotherapy.

Original languageEnglish (US)
Pages (from-to)8037-8045
Number of pages9
JournalJournal of Immunology
Volume177
Issue number11
DOIs
StatePublished - Dec 1 2006

Fingerprint

Papillomavirus Infections
Epitopes
Immunity
Rabbits
Cottontail rabbit papillomavirus
DNA Vaccines
Human papillomavirus 16
Virus Diseases
Immunotherapy
Cultured Cells
Software
Spleen
Animal Models
Clone Cells
Viruses
T-Lymphocytes
Amino Acids
DNA
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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title = "An HLA-A2.1-transgenic rabbit model to study immunity to papillomavirus infection",
abstract = "We have established several HLA-A2.1-transgenic rabbit lines to provide a host to study CD8+ T cell responses during virus infections. HLA-A2.1 protein expression was detected on cell surfaces within various organ tissues. Continuous cultured cells from these transgenic rabbits were capable of presenting both endogenous and exogenous HLA-A2.1-restricted epitopes to an HLA-A2.1-restricted epitope-specific CTL clone. A DNA vaccine containing an HLA-A2.1-restricted human papillomavirus type 16 E7 epitope (amino acid residues 82-90) stimulated epitope-specific CTLs in both PBLs and spleen cells of transgenic rabbits. In addition, vaccinated transgenic rabbits were protected against infection with a mutant cottontail rabbit papillomavirus DNA containing an embedded human papillomavirus type 16 E7/82-90 epitope. Complete protection was achieved using a multivalent epitope DNA vaccine based on epitope selection from cottontail rabbit papillomavirus E1 using MHC class I epitope prediction software. HLA-A2.1-transgenic rabbits will be an important preclinical animal model system to study virus-host interactions and to assess specific targets for immunotherapy.",
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An HLA-A2.1-transgenic rabbit model to study immunity to papillomavirus infection. / Hu, Jiafen; Peng, Xuwen; Schell, Todd D.; Budgeon, Lynn R.; Cladel, Nancy M.; Christensen, Neil D.

In: Journal of Immunology, Vol. 177, No. 11, 01.12.2006, p. 8037-8045.

Research output: Contribution to journalArticle

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AU - Christensen, Neil D.

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