An ice nucleation reporter gene system: Identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola

P. B. Lindgren, R. Frederick, A. G. Govindarajan, N. J. Panopoulos, B. J. Staskawicz, S. E. Lindow

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We have constructed derivatives of the transposon Tn3 that allow an ice nucleation gene (inaZ) to be used as 'reporter' of the transcriptional activity of genes into which it is inserted. In these derivatives (Tn3-Ice and Tn3-Spice), the lacZYA sequences of transposon Tn3-HoHo1 were replaced with inaZ lacking its native promoter. The ice nucleation activity of virB::inaZ fusions in the correct transcriptional orientation was inducible by acetosyringone, a plant metabolite which activates the vir operon of Agrobacterium tumefaciens Ti plasmids, while fusions in the opposite orientation were unresponsive to the inducer. Tn3-Spice was also used to investigate the expression of a cluster of genes (hrp) which control pathogenicity and hypersensitivity elicited by Pseudomonas syringae pv. phaseolicola. An inducible region was identified which is expressed at low levels in vitro but becomes activated when the bacteria come into contact with the susceptible host, bean. Activation of this region occurred within 2 h post-inoculation and was nearly complete by the time the bacteria began to multiply in the leaf tissue. The inaZ reporter appears to be at least 105-fold more sensitive than lacZ in P.s.phaseolicola. Thus, the inaZ fusion system provides a sensitive, convenient and inexpensive tool for the study of bacterial gene expression, particularly during plant pathogenesis, and should be generally useful as a reporter gene system in Gram-negative bacteria.

Original languageEnglish (US)
Pages (from-to)1291-1301
Number of pages11
JournalEMBO Journal
Volume8
Issue number5
StatePublished - Jan 1 1989

Fingerprint

Pseudomonas syringae
Ice
Reporter Genes
Virulence
Spices
Identification (control systems)
Nucleation
Genes
Bacteria
Fusion reactions
Plant Tumor-Inducing Plasmids
Bacterial Genes
Operon
Multigene Family
Derivatives
Gram-Negative Bacteria
Hypersensitivity
Metabolites
Gene expression
Gene Expression

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Lindgren, P. B., Frederick, R., Govindarajan, A. G., Panopoulos, N. J., Staskawicz, B. J., & Lindow, S. E. (1989). An ice nucleation reporter gene system: Identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. EMBO Journal, 8(5), 1291-1301.
Lindgren, P. B. ; Frederick, R. ; Govindarajan, A. G. ; Panopoulos, N. J. ; Staskawicz, B. J. ; Lindow, S. E. / An ice nucleation reporter gene system : Identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. In: EMBO Journal. 1989 ; Vol. 8, No. 5. pp. 1291-1301.
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Lindgren, PB, Frederick, R, Govindarajan, AG, Panopoulos, NJ, Staskawicz, BJ & Lindow, SE 1989, 'An ice nucleation reporter gene system: Identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola', EMBO Journal, vol. 8, no. 5, pp. 1291-1301.

An ice nucleation reporter gene system : Identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. / Lindgren, P. B.; Frederick, R.; Govindarajan, A. G.; Panopoulos, N. J.; Staskawicz, B. J.; Lindow, S. E.

In: EMBO Journal, Vol. 8, No. 5, 01.01.1989, p. 1291-1301.

Research output: Contribution to journalArticle

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AU - Lindgren, P. B.

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Lindgren PB, Frederick R, Govindarajan AG, Panopoulos NJ, Staskawicz BJ, Lindow SE. An ice nucleation reporter gene system: Identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. EMBO Journal. 1989 Jan 1;8(5):1291-1301.