An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi

Fulya Baysal-Gurel, Melanie L. Lewis Ivey, Anne Dorrance, Douglas Luster, Reid David Frederick, Jill Czarnecki, Michael Boehm, Sally A. Miller

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact non-germinated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006.

Original languageEnglish (US)
Pages (from-to)1387-1393
Number of pages7
JournalPlant disease
Volume92
Issue number10
DOIs
StatePublished - Oct 1 2008

Fingerprint

Phakopsora pachyrhizi
fluorescent antibody technique
antiserum
spores
assays
microscopes
glass
Phakopsora
petrolatum
rabbits
soybeans
samplers
coatings
goats
enzyme-linked immunosorbent assay
air
urediniospores
pathogens
leaves

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Baysal-Gurel, F., Lewis Ivey, M. L., Dorrance, A., Luster, D., Frederick, R. D., Czarnecki, J., ... Miller, S. A. (2008). An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi. Plant disease, 92(10), 1387-1393. https://doi.org/10.1094/PDIS-92-10-1387
Baysal-Gurel, Fulya ; Lewis Ivey, Melanie L. ; Dorrance, Anne ; Luster, Douglas ; Frederick, Reid David ; Czarnecki, Jill ; Boehm, Michael ; Miller, Sally A. / An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi. In: Plant disease. 2008 ; Vol. 92, No. 10. pp. 1387-1393.
@article{ac5cc5feb7634e3da96ef15055bb99af,
title = "An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi",
abstract = "An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact non-germinated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006.",
author = "Fulya Baysal-Gurel and {Lewis Ivey}, {Melanie L.} and Anne Dorrance and Douglas Luster and Frederick, {Reid David} and Jill Czarnecki and Michael Boehm and Miller, {Sally A.}",
year = "2008",
month = "10",
day = "1",
doi = "10.1094/PDIS-92-10-1387",
language = "English (US)",
volume = "92",
pages = "1387--1393",
journal = "Plant Disease",
issn = "0191-2917",
publisher = "American Phytopathological Society",
number = "10",

}

Baysal-Gurel, F, Lewis Ivey, ML, Dorrance, A, Luster, D, Frederick, RD, Czarnecki, J, Boehm, M & Miller, SA 2008, 'An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi', Plant disease, vol. 92, no. 10, pp. 1387-1393. https://doi.org/10.1094/PDIS-92-10-1387

An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi. / Baysal-Gurel, Fulya; Lewis Ivey, Melanie L.; Dorrance, Anne; Luster, Douglas; Frederick, Reid David; Czarnecki, Jill; Boehm, Michael; Miller, Sally A.

In: Plant disease, Vol. 92, No. 10, 01.10.2008, p. 1387-1393.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi

AU - Baysal-Gurel, Fulya

AU - Lewis Ivey, Melanie L.

AU - Dorrance, Anne

AU - Luster, Douglas

AU - Frederick, Reid David

AU - Czarnecki, Jill

AU - Boehm, Michael

AU - Miller, Sally A.

PY - 2008/10/1

Y1 - 2008/10/1

N2 - An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact non-germinated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006.

AB - An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact non-germinated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006.

UR - http://www.scopus.com/inward/record.url?scp=55549138772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55549138772&partnerID=8YFLogxK

U2 - 10.1094/PDIS-92-10-1387

DO - 10.1094/PDIS-92-10-1387

M3 - Article

AN - SCOPUS:55549138772

VL - 92

SP - 1387

EP - 1393

JO - Plant Disease

JF - Plant Disease

SN - 0191-2917

IS - 10

ER -

Baysal-Gurel F, Lewis Ivey ML, Dorrance A, Luster D, Frederick RD, Czarnecki J et al. An immunofluorescence assay to detect urediniospores of Phakopsora pachyrhizi. Plant disease. 2008 Oct 1;92(10):1387-1393. https://doi.org/10.1094/PDIS-92-10-1387