An improved histochemical method for measuring nitric oxide synthase activity

Jamshid Alaghband-Zadeh, Siroos Mehdizadeh, Anne O'Farrell, Judith Weisz, Lucille Bitensky, Joseph Chayen

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The previous quantitative histochemical method for measuring nitric oxide synthase (NOS) activity in tissue sections involved the loss of about 15 per cent of the NOS, presumably from the section into the reaction medium. Two changes are now described. The first is concerned with the preparation in the laboratory of the active reagent, lead ammonium citrate/acetate (LACA). The second change involves an improvement of the procedure for measuring NOS activity. The new method appears to retain all the measurable NOS activity inside the section.

Original languageEnglish (US)
Pages (from-to)217-220
Number of pages4
JournalCell Biochemistry and Function
Volume17
Issue number3
DOIs
StatePublished - Sep 1 1999

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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