TY - JOUR
T1 - An improved histochemical method for measuring nitric oxide synthase activity
AU - Alaghband-Zadeh, Jamshid
AU - Mehdizadeh, Siroos
AU - O'Farrell, Anne
AU - Weisz, Judith
AU - Bitensky, Lucille
AU - Chayen, Joseph
PY - 1999/9/1
Y1 - 1999/9/1
N2 - The previous quantitative histochemical method for measuring nitric oxide synthase (NOS) activity in tissue sections involved the loss of about 15 per cent of the NOS, presumably from the section into the reaction medium. Two changes are now described. The first is concerned with the preparation in the laboratory of the active reagent, lead ammonium citrate/acetate (LACA). The second change involves an improvement of the procedure for measuring NOS activity. The new method appears to retain all the measurable NOS activity inside the section.
AB - The previous quantitative histochemical method for measuring nitric oxide synthase (NOS) activity in tissue sections involved the loss of about 15 per cent of the NOS, presumably from the section into the reaction medium. Two changes are now described. The first is concerned with the preparation in the laboratory of the active reagent, lead ammonium citrate/acetate (LACA). The second change involves an improvement of the procedure for measuring NOS activity. The new method appears to retain all the measurable NOS activity inside the section.
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U2 - 10.1002/(SICI)1099-0844(199909)17:3<217::AID-CBF826>3.0.CO;2-M
DO - 10.1002/(SICI)1099-0844(199909)17:3<217::AID-CBF826>3.0.CO;2-M
M3 - Article
C2 - 10451544
AN - SCOPUS:0032821445
SN - 0263-6484
VL - 17
SP - 217
EP - 220
JO - Cell Biochemistry and Function
JF - Cell Biochemistry and Function
IS - 3
ER -