An improved method for isolating RNA from porcine adipose tissue

I. Louveau, S. Chaudhuri, Terry D. Etherton

Research output: Contribution to journalArticle

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Abstract

In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality.

Original languageEnglish (US)
Pages (from-to)308-310
Number of pages3
JournalAnalytical Biochemistry
Volume196
Issue number2
DOIs
Publication statusPublished - Aug 1 1991

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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