In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology