An in vitro system for studying the initial stages of cottontail rabbit papillomavirus infection

Michael G. Angell, Neil Christensen, John W. Kreider

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cottontail rabbit papillomavirus (CRPV) infection of an established cottontail epidermal cell line (Sf1 Ep) resulted in the production of CRPV-specific transcripts without concomitant morphological transformation. The most abundant transcripts corresponded in size to those of the E6 and E7 open reading frames (ORFs), which are also among the commonest in domestic and cottontail rabbit papillomas. CRPV RNA production was both time- and dose-dependent, with RNA production diminishing with decreasing viral dose and increasing culture passage. Infected cultures contained episomal CRPV DNA, which did not appreciably change in abundance with time but was significantly reduced with culture passage. All features of in vitro infection, especially RNA production, were inhibited by CRPV-neutralizing, but not HPV-11-neutralizing, monoclonal antibodies. Much of this inhibition could be attributed to a blockage of viral penetration, as indicated by the reduction of CRPV DNA within virus-neutralized cultures. The results indicate that, although CRPV infection of Sf1 Ep cells was abortive, it serves as a useful model for analysis of early infection events.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalJournal of Virological Methods
Volume39
Issue number1-2
DOIs
StatePublished - Jan 1 1992

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Cottontail rabbit papillomavirus
Papillomavirus Infections
RNA
Human papillomavirus 11
DNA Viruses
Papilloma
Infection
In Vitro Techniques
Neutralizing Antibodies
Open Reading Frames
Monoclonal Antibodies
Rabbits
Cell Line

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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abstract = "Cottontail rabbit papillomavirus (CRPV) infection of an established cottontail epidermal cell line (Sf1 Ep) resulted in the production of CRPV-specific transcripts without concomitant morphological transformation. The most abundant transcripts corresponded in size to those of the E6 and E7 open reading frames (ORFs), which are also among the commonest in domestic and cottontail rabbit papillomas. CRPV RNA production was both time- and dose-dependent, with RNA production diminishing with decreasing viral dose and increasing culture passage. Infected cultures contained episomal CRPV DNA, which did not appreciably change in abundance with time but was significantly reduced with culture passage. All features of in vitro infection, especially RNA production, were inhibited by CRPV-neutralizing, but not HPV-11-neutralizing, monoclonal antibodies. Much of this inhibition could be attributed to a blockage of viral penetration, as indicated by the reduction of CRPV DNA within virus-neutralized cultures. The results indicate that, although CRPV infection of Sf1 Ep cells was abortive, it serves as a useful model for analysis of early infection events.",
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An in vitro system for studying the initial stages of cottontail rabbit papillomavirus infection. / Angell, Michael G.; Christensen, Neil; Kreider, John W.

In: Journal of Virological Methods, Vol. 39, No. 1-2, 01.01.1992, p. 207-216.

Research output: Contribution to journalArticle

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