An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells

Michael Ohh, William Y. Kim, Javid J. Moslehi, Yuzhi Chen, Vincent Chau, Margaret A. Read, William G. Kaelin

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Skp1-Cdc53/Cul1-F-box (SCF) complexes constitute a class of E3 ubiquitin ligases. Recently, a multiprotein complex containing pVHL, elongin C and Cul2 (VEC) was shown to structurally and functionally resemble SCF complexes. Cdc53 and the Cullins can become covalently linked to the ubiquitin-like molecule Rub1/NEDD8. Inhibition of neddylation inhibits SCF function in vitro and in yeast and plants. Here we show that ongoing neddylation is likewise required for VEC function in vitro and for the degradation of SCF and VEC targets in mammalian cells. Thus, neddylation regulates the activity of two specific subclasses of mammalian ubiquitin ligases.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalEMBO Reports
Volume3
Issue number2
DOIs
StatePublished - Jan 1 2002

Fingerprint

Cullin Proteins
Ubiquitination
Ubiquitin
Cells
Multiprotein Complexes
Ubiquitin-Protein Ligases
Ligases
Yeast
Yeasts
Degradation
Molecules
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Ohh, Michael ; Kim, William Y. ; Moslehi, Javid J. ; Chen, Yuzhi ; Chau, Vincent ; Read, Margaret A. ; Kaelin, William G. / An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells. In: EMBO Reports. 2002 ; Vol. 3, No. 2. pp. 177-182.
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An intact NEDD8 pathway is required for Cullin-dependent ubiquitylation in mammalian cells. / Ohh, Michael; Kim, William Y.; Moslehi, Javid J.; Chen, Yuzhi; Chau, Vincent; Read, Margaret A.; Kaelin, William G.

In: EMBO Reports, Vol. 3, No. 2, 01.01.2002, p. 177-182.

Research output: Contribution to journalArticle

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