An integrated genetic map of Populus deltoides based on amplified fragment length polymorphisms

Rongling Wu, Y. F. Han, J. J. Hu, J. J. Fang, L. Li, M. L. Li, Z. B. Zeng

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Amplified fragment length polymorphism (AFLP) is an efficient molecular technique for generating a large number of DNA-based genetic markers in Populus. We have constructed an integrated genetic map for a Populus backcross population derived from two selected P. deltoides clones using AFLP markers. A traditional strategy for genetic mapping in outcrossing species, such as forest trees, is based on two-way pseudotestcross configurations of the markers (testcross markers) heterozygous in one parent and null in the other. By using the markers segregating in both parents (intercross markers) as bridges, the two parent-specific genetic maps can be aligned. In this study, we detected a number of non-parental heteroduplex markers resulting from the PCR amplification of two DNA segments that have a high degree of homology to one another but differ in their nucleotide sequences. These heteroduplex markers detected have served as bridges to generate an integrated map which includes 19 major linkage groups equal to the Populus haploid chromosome number and 24 minor groups. The 19 major linkage groups cover a total of 2,927 cM, with an average spacing between two markers of 23.3 cM. The map developed in this study provides a first step in producing a highly saturated linkage map of the Populus deltoides genome.

Original languageEnglish (US)
Pages (from-to)1249-1256
Number of pages8
JournalTheoretical and Applied Genetics
Volume100
Issue number8
DOIs
StatePublished - Jan 1 2000

Fingerprint

Populus
Populus deltoides
amplified fragment length polymorphism
linkage groups
chromosome mapping
DNA
outcrossing
forest trees
haploidy
chromosome number
Haploidy
Genetic Markers
spatial distribution
clones
nucleotide sequences
genetic markers
genome
Clone Cells
Chromosomes
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Wu, Rongling ; Han, Y. F. ; Hu, J. J. ; Fang, J. J. ; Li, L. ; Li, M. L. ; Zeng, Z. B. / An integrated genetic map of Populus deltoides based on amplified fragment length polymorphisms. In: Theoretical and Applied Genetics. 2000 ; Vol. 100, No. 8. pp. 1249-1256.
@article{b255daa726044cd3a4ae218ab673a206,
title = "An integrated genetic map of Populus deltoides based on amplified fragment length polymorphisms",
abstract = "Amplified fragment length polymorphism (AFLP) is an efficient molecular technique for generating a large number of DNA-based genetic markers in Populus. We have constructed an integrated genetic map for a Populus backcross population derived from two selected P. deltoides clones using AFLP markers. A traditional strategy for genetic mapping in outcrossing species, such as forest trees, is based on two-way pseudotestcross configurations of the markers (testcross markers) heterozygous in one parent and null in the other. By using the markers segregating in both parents (intercross markers) as bridges, the two parent-specific genetic maps can be aligned. In this study, we detected a number of non-parental heteroduplex markers resulting from the PCR amplification of two DNA segments that have a high degree of homology to one another but differ in their nucleotide sequences. These heteroduplex markers detected have served as bridges to generate an integrated map which includes 19 major linkage groups equal to the Populus haploid chromosome number and 24 minor groups. The 19 major linkage groups cover a total of 2,927 cM, with an average spacing between two markers of 23.3 cM. The map developed in this study provides a first step in producing a highly saturated linkage map of the Populus deltoides genome.",
author = "Rongling Wu and Han, {Y. F.} and Hu, {J. J.} and Fang, {J. J.} and L. Li and Li, {M. L.} and Zeng, {Z. B.}",
year = "2000",
month = "1",
day = "1",
doi = "10.1007/s001220051431",
language = "English (US)",
volume = "100",
pages = "1249--1256",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "8",

}

An integrated genetic map of Populus deltoides based on amplified fragment length polymorphisms. / Wu, Rongling; Han, Y. F.; Hu, J. J.; Fang, J. J.; Li, L.; Li, M. L.; Zeng, Z. B.

In: Theoretical and Applied Genetics, Vol. 100, No. 8, 01.01.2000, p. 1249-1256.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An integrated genetic map of Populus deltoides based on amplified fragment length polymorphisms

AU - Wu, Rongling

AU - Han, Y. F.

AU - Hu, J. J.

AU - Fang, J. J.

AU - Li, L.

AU - Li, M. L.

AU - Zeng, Z. B.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Amplified fragment length polymorphism (AFLP) is an efficient molecular technique for generating a large number of DNA-based genetic markers in Populus. We have constructed an integrated genetic map for a Populus backcross population derived from two selected P. deltoides clones using AFLP markers. A traditional strategy for genetic mapping in outcrossing species, such as forest trees, is based on two-way pseudotestcross configurations of the markers (testcross markers) heterozygous in one parent and null in the other. By using the markers segregating in both parents (intercross markers) as bridges, the two parent-specific genetic maps can be aligned. In this study, we detected a number of non-parental heteroduplex markers resulting from the PCR amplification of two DNA segments that have a high degree of homology to one another but differ in their nucleotide sequences. These heteroduplex markers detected have served as bridges to generate an integrated map which includes 19 major linkage groups equal to the Populus haploid chromosome number and 24 minor groups. The 19 major linkage groups cover a total of 2,927 cM, with an average spacing between two markers of 23.3 cM. The map developed in this study provides a first step in producing a highly saturated linkage map of the Populus deltoides genome.

AB - Amplified fragment length polymorphism (AFLP) is an efficient molecular technique for generating a large number of DNA-based genetic markers in Populus. We have constructed an integrated genetic map for a Populus backcross population derived from two selected P. deltoides clones using AFLP markers. A traditional strategy for genetic mapping in outcrossing species, such as forest trees, is based on two-way pseudotestcross configurations of the markers (testcross markers) heterozygous in one parent and null in the other. By using the markers segregating in both parents (intercross markers) as bridges, the two parent-specific genetic maps can be aligned. In this study, we detected a number of non-parental heteroduplex markers resulting from the PCR amplification of two DNA segments that have a high degree of homology to one another but differ in their nucleotide sequences. These heteroduplex markers detected have served as bridges to generate an integrated map which includes 19 major linkage groups equal to the Populus haploid chromosome number and 24 minor groups. The 19 major linkage groups cover a total of 2,927 cM, with an average spacing between two markers of 23.3 cM. The map developed in this study provides a first step in producing a highly saturated linkage map of the Populus deltoides genome.

UR - http://www.scopus.com/inward/record.url?scp=0033947565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033947565&partnerID=8YFLogxK

U2 - 10.1007/s001220051431

DO - 10.1007/s001220051431

M3 - Article

AN - SCOPUS:0033947565

VL - 100

SP - 1249

EP - 1256

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 8

ER -