An ionic liquid form of DNA

Redox-active molten salts of nucleic acids

A. M. Leone, S. C. Weatherly, M. E. Williams, H. H. Thorp, R. W. Murray

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Ionic liquids are described that contain duplex DNA as the anion and polyether-decorated transition metal complexes based on M(MePEG-bpy)32+ as the cation (M = Fe, Co; MePEG-bpy = 4,4′-(CH3(OCH2 -CH2)7OCO)2-2,2′-bipyridine). When the undiluted liquid DNA - or molten salt - is interrogated electrochemically by a microelectrode, the molten salts exhibit cyclic voltammograms due to the physical diffusion (DPHYS) of the polyether-transition metal complex. When M = Co(II), the cyclic voltammogram of the melt shows an oxidative wave due to the Co(III/II) couple at E1/2 = 0.40 V (versus Ag/AgCl) and a DPHYS of 6 × 10-12 cm2/s, which is significantly lower than that for Co(MePEG-bpy)3(ClO4)2 (DPHYS = 2.6 × 10-10 cm2/s) due to greater viscosity provoked by the DNA polymer. When a 1:1 mixture is made of the Co(MePEG-bpy)3. DNA and Fe(MePEG-bpy)3(ClO4)2 melts, two redox waves are observed. The first is due to the Co(III/II) couple, and the second is a catalytic wave due to oxidation of guanine in DNA by electrogenerated Fe(III) in the undiluted melt. Independent experiments show that the Fe(III) form of the complex selectively oxidizes guanine in duplex DNA. These DNA molten salts constitute a new class of materials whose properties can be controlled by nucleic acid sequence and that can be interrogated in undiluted form on microelectrode arrays.

Original languageEnglish (US)
Pages (from-to)218-222
Number of pages5
JournalJournal of the American Chemical Society
Volume123
Issue number2
DOIs
StatePublished - Jan 17 2001

Fingerprint

Ionic Liquids
Nucleic acids
Ionic liquids
Nucleic Acids
Oxidation-Reduction
Molten materials
DNA
Salts
Polyethers
Microelectrodes
Coordination Complexes
Guanine
Metal complexes
Transition metals
Nucleic acid sequences
Viscosity
Anions
Cations
Materials properties
Polymers

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Leone, A. M. ; Weatherly, S. C. ; Williams, M. E. ; Thorp, H. H. ; Murray, R. W. / An ionic liquid form of DNA : Redox-active molten salts of nucleic acids. In: Journal of the American Chemical Society. 2001 ; Vol. 123, No. 2. pp. 218-222.
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abstract = "Ionic liquids are described that contain duplex DNA as the anion and polyether-decorated transition metal complexes based on M(MePEG-bpy)32+ as the cation (M = Fe, Co; MePEG-bpy = 4,4′-(CH3(OCH2 -CH2)7OCO)2-2,2′-bipyridine). When the undiluted liquid DNA - or molten salt - is interrogated electrochemically by a microelectrode, the molten salts exhibit cyclic voltammograms due to the physical diffusion (DPHYS) of the polyether-transition metal complex. When M = Co(II), the cyclic voltammogram of the melt shows an oxidative wave due to the Co(III/II) couple at E1/2 = 0.40 V (versus Ag/AgCl) and a DPHYS of 6 × 10-12 cm2/s, which is significantly lower than that for Co(MePEG-bpy)3(ClO4)2 (DPHYS = 2.6 × 10-10 cm2/s) due to greater viscosity provoked by the DNA polymer. When a 1:1 mixture is made of the Co(MePEG-bpy)3. DNA and Fe(MePEG-bpy)3(ClO4)2 melts, two redox waves are observed. The first is due to the Co(III/II) couple, and the second is a catalytic wave due to oxidation of guanine in DNA by electrogenerated Fe(III) in the undiluted melt. Independent experiments show that the Fe(III) form of the complex selectively oxidizes guanine in duplex DNA. These DNA molten salts constitute a new class of materials whose properties can be controlled by nucleic acid sequence and that can be interrogated in undiluted form on microelectrode arrays.",
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An ionic liquid form of DNA : Redox-active molten salts of nucleic acids. / Leone, A. M.; Weatherly, S. C.; Williams, M. E.; Thorp, H. H.; Murray, R. W.

In: Journal of the American Chemical Society, Vol. 123, No. 2, 17.01.2001, p. 218-222.

Research output: Contribution to journalArticle

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T2 - Redox-active molten salts of nucleic acids

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AU - Murray, R. W.

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N2 - Ionic liquids are described that contain duplex DNA as the anion and polyether-decorated transition metal complexes based on M(MePEG-bpy)32+ as the cation (M = Fe, Co; MePEG-bpy = 4,4′-(CH3(OCH2 -CH2)7OCO)2-2,2′-bipyridine). When the undiluted liquid DNA - or molten salt - is interrogated electrochemically by a microelectrode, the molten salts exhibit cyclic voltammograms due to the physical diffusion (DPHYS) of the polyether-transition metal complex. When M = Co(II), the cyclic voltammogram of the melt shows an oxidative wave due to the Co(III/II) couple at E1/2 = 0.40 V (versus Ag/AgCl) and a DPHYS of 6 × 10-12 cm2/s, which is significantly lower than that for Co(MePEG-bpy)3(ClO4)2 (DPHYS = 2.6 × 10-10 cm2/s) due to greater viscosity provoked by the DNA polymer. When a 1:1 mixture is made of the Co(MePEG-bpy)3. DNA and Fe(MePEG-bpy)3(ClO4)2 melts, two redox waves are observed. The first is due to the Co(III/II) couple, and the second is a catalytic wave due to oxidation of guanine in DNA by electrogenerated Fe(III) in the undiluted melt. Independent experiments show that the Fe(III) form of the complex selectively oxidizes guanine in duplex DNA. These DNA molten salts constitute a new class of materials whose properties can be controlled by nucleic acid sequence and that can be interrogated in undiluted form on microelectrode arrays.

AB - Ionic liquids are described that contain duplex DNA as the anion and polyether-decorated transition metal complexes based on M(MePEG-bpy)32+ as the cation (M = Fe, Co; MePEG-bpy = 4,4′-(CH3(OCH2 -CH2)7OCO)2-2,2′-bipyridine). When the undiluted liquid DNA - or molten salt - is interrogated electrochemically by a microelectrode, the molten salts exhibit cyclic voltammograms due to the physical diffusion (DPHYS) of the polyether-transition metal complex. When M = Co(II), the cyclic voltammogram of the melt shows an oxidative wave due to the Co(III/II) couple at E1/2 = 0.40 V (versus Ag/AgCl) and a DPHYS of 6 × 10-12 cm2/s, which is significantly lower than that for Co(MePEG-bpy)3(ClO4)2 (DPHYS = 2.6 × 10-10 cm2/s) due to greater viscosity provoked by the DNA polymer. When a 1:1 mixture is made of the Co(MePEG-bpy)3. DNA and Fe(MePEG-bpy)3(ClO4)2 melts, two redox waves are observed. The first is due to the Co(III/II) couple, and the second is a catalytic wave due to oxidation of guanine in DNA by electrogenerated Fe(III) in the undiluted melt. Independent experiments show that the Fe(III) form of the complex selectively oxidizes guanine in duplex DNA. These DNA molten salts constitute a new class of materials whose properties can be controlled by nucleic acid sequence and that can be interrogated in undiluted form on microelectrode arrays.

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