An iron responsive element-like stem-loop regulates α-hemoglobin- stabilizing protein mRNA

Camila O. Dos Santos, Louis C. Dore, Eric Valentine, Suresh G. Shelat, Ross C. Hardison, Manik Ghosh, Wei Wang, Richard S. Eisenstein, Fernando F. Costa, Mitchell J. Weiss

Research output: Contribution to journalArticle

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Abstract

Hemoglobin production during erythropoiesis is mechanistically coupled to the acquisition and metabolism of iron. We discovered that iron regulates the expression of α-hemoglobin-stabilizing protein (AHSP), a molecular chaperone that binds and stabilizes free α-globin during hemoglobin synthesis. In primates, the 3′-untranslated region (UTR) of AHSP mRNA contains a nucleotide sequence resembling iron responsive elements (IREs), stem-loop structures that regulate gene expression post-transcriptionally by binding iron regulatory proteins (IRPs). The AHSP IRE-like stem-loop deviates from classical consensus sequences and binds IRPs poorly in electrophoretic mobility shift assays. However, in cytoplasmic extracts, AHSP mRNA co-immunoprecipitates with IRPs in a fashion that is dependent on the stem-loop structure and inhibited by iron. Moreover, this interaction enhances AHSP mRNA stability in erythroid and heterologous cells. Our findings demonstrate that IRPs can regulate mRNA expression through non-canonical IREs and extend the repertoire of known iron-regulated genes. In addition, we illustrate a new mechanism through which hemoglobin may be modulated according to iron status.

Original languageEnglish (US)
Pages (from-to)26956-26964
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number40
DOIs
StatePublished - Oct 3 2008

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Hemoglobins
Iron
Iron-Regulatory Proteins
Messenger RNA
Proteins
Electrophoretic mobility
Erythroid Cells
Molecular Chaperones
Globins
Erythropoiesis
RNA Stability
Consensus Sequence
3' Untranslated Regions
Electrophoretic Mobility Shift Assay
Metabolism
Gene expression
Primates
Assays
Carrier Proteins
Nucleotides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dos Santos, C. O., Dore, L. C., Valentine, E., Shelat, S. G., Hardison, R. C., Ghosh, M., ... Weiss, M. J. (2008). An iron responsive element-like stem-loop regulates α-hemoglobin- stabilizing protein mRNA. Journal of Biological Chemistry, 283(40), 26956-26964. https://doi.org/10.1074/jbc.M802421200
Dos Santos, Camila O. ; Dore, Louis C. ; Valentine, Eric ; Shelat, Suresh G. ; Hardison, Ross C. ; Ghosh, Manik ; Wang, Wei ; Eisenstein, Richard S. ; Costa, Fernando F. ; Weiss, Mitchell J. / An iron responsive element-like stem-loop regulates α-hemoglobin- stabilizing protein mRNA. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 40. pp. 26956-26964.
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Dos Santos, CO, Dore, LC, Valentine, E, Shelat, SG, Hardison, RC, Ghosh, M, Wang, W, Eisenstein, RS, Costa, FF & Weiss, MJ 2008, 'An iron responsive element-like stem-loop regulates α-hemoglobin- stabilizing protein mRNA', Journal of Biological Chemistry, vol. 283, no. 40, pp. 26956-26964. https://doi.org/10.1074/jbc.M802421200

An iron responsive element-like stem-loop regulates α-hemoglobin- stabilizing protein mRNA. / Dos Santos, Camila O.; Dore, Louis C.; Valentine, Eric; Shelat, Suresh G.; Hardison, Ross C.; Ghosh, Manik; Wang, Wei; Eisenstein, Richard S.; Costa, Fernando F.; Weiss, Mitchell J.

In: Journal of Biological Chemistry, Vol. 283, No. 40, 03.10.2008, p. 26956-26964.

Research output: Contribution to journalArticle

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AU - Dore, Louis C.

AU - Valentine, Eric

AU - Shelat, Suresh G.

AU - Hardison, Ross C.

AU - Ghosh, Manik

AU - Wang, Wei

AU - Eisenstein, Richard S.

AU - Costa, Fernando F.

AU - Weiss, Mitchell J.

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