An NMR based phosphodiesterase assay

Madoka Akimoto, Tianning Yu, Kody Moleschi, Katherine Van, Ganesh S. Anand, Giuseppe Melacini

Research output: Contribution to journalArticlepeer-review

Abstract

We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors. This journal is

Original languageEnglish (US)
Pages (from-to)8091-8094
Number of pages4
JournalChemical Communications
Volume56
Issue number58
DOIs
StatePublished - Jul 25 2020

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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