An oral glucose load decreases postprandial extracellular vesicles in obese adults with and without prediabetes

Natalie Z.M. Eichner, Nicole M. Gilbertson, Luca Musante, Sabrina La Salvia, Arthur Weltman, Uta Erdbrügger, Steven K. Malin

Research output: Contribution to journalArticle

Abstract

Although extracellular vesicles (EVs) are a novel biomediator of type 2 diabetes (T2D) and cardiovascular disease (CVD), the effects of hyperglycemia on EVs in humans is unknown. We tested the hypothesis that a 75-g oral glucose tolerance test (OGTT) would promote changes in EVs in relation to CVD risk. Twenty-five obese adults (Age: 52.4 ± 3.2 year, BMI: 32.5 ± 1.2 kg/m2) were screened for normal glucose tolerance (NGT, n = 8) and prediabetes (PD, n = 17) using American Diabetes Association criteria (75 g OGTT and/or HbA1c). Body composition (bioelectrical impedance) and fitness (VO2peak) were measured. Arterial stiffness (augmentation index; AIx) was measured at 0, 60- and 120-min while insulin, glucose, and free fatty acids were evaluated every 30 min during the OGTT to assess CVD risk. Annexin V positive (AV+) and Annexin V negative (AV−) total EVs, platelet EVs (CD31+/CD41+; CD41+), leukocyte EVs (CD45+; CD45+/CD41−), platelet endothelial cell adhesion molecule (PECAM) (CD31+) and endothelial EVs (CD 31+/CD41; CD105+) were collected at 0 and 120 min. There were no differences in age, BMI, or body fat between NGT and PD (all P > 0.63). Total EVs, AV+ CD31+ (PECAM), and AV+ CD31+/CD41 (endothelial) EVs decreased after the OGTT (P ≤ 0.04). Circulating insulin at 2-h correlated with elevated post-prandial AV− CD45+ (r = 0.48, P = 0.04) while arterial stiffness related to reduced total EVs (r = −0.49, P = 0.03) and AV+ CD41+ (platelet) (r = −0.52, P = 0.02). An oral glucose load lowers post-prandial total, platelet, and endothelial EVs in obese adults with NGT and prediabetes in relation to CVD risk.

Original languageEnglish (US)
Article number580
JournalNutrients
Volume11
Issue number3
DOIs
StatePublished - Mar 2019

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Prediabetic State
cardiovascular diseases
mouth
Annexin A5
Glucose
glucose
cell adhesion
endothelial cells
insulin
Glucose Tolerance Test
Blood Platelets
glycohemoglobin
bioelectrical impedance
Cardiovascular Diseases
glucose tolerance
hyperglycemia
noninsulin-dependent diabetes mellitus
body fat
body composition
Vascular Stiffness

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

Cite this

Eichner, N. Z. M., Gilbertson, N. M., Musante, L., La Salvia, S., Weltman, A., Erdbrügger, U., & Malin, S. K. (2019). An oral glucose load decreases postprandial extracellular vesicles in obese adults with and without prediabetes. Nutrients, 11(3), [580]. https://doi.org/10.3390/nu11030580
Eichner, Natalie Z.M. ; Gilbertson, Nicole M. ; Musante, Luca ; La Salvia, Sabrina ; Weltman, Arthur ; Erdbrügger, Uta ; Malin, Steven K. / An oral glucose load decreases postprandial extracellular vesicles in obese adults with and without prediabetes. In: Nutrients. 2019 ; Vol. 11, No. 3.
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An oral glucose load decreases postprandial extracellular vesicles in obese adults with and without prediabetes. / Eichner, Natalie Z.M.; Gilbertson, Nicole M.; Musante, Luca; La Salvia, Sabrina; Weltman, Arthur; Erdbrügger, Uta; Malin, Steven K.

In: Nutrients, Vol. 11, No. 3, 580, 03.2019.

Research output: Contribution to journalArticle

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AU - Gilbertson, Nicole M.

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