@article{234690baba7641929f02a4803947839c,
title = "An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2",
abstract = "We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.",
author = "Jadranka Milosevic and Mengrou Lu and Wallace Greene and He, {Hong Zhang} and Zheng, {Si Yang}",
note = "Funding Information: We thank Dr. Florent Letronne from SARS-CoV-2 testing laboratory at Carnegie Mellon University for providing us access to QuantStudio 7 Flex real-time PCR systems, and Sheng Zhang from the Biomedical Engineering Department at Carnegie Mellon University for performing the validation of the ultrafast one-step qRT-PCR assay protocol. This work was financially supported National Institutes of Health (NIH/NIAID 7R01AI134911 and NIH/NIAID 1R43AI145614-01). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Milosevic, Lu, Greene, He and Zheng.",
year = "2021",
month = nov,
day = "4",
doi = "10.3389/fmicb.2021.749783",
language = "English (US)",
volume = "12",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S. A.",
}