An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner

Jennelle L. Malcos, Richard J. Cyr

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Past phylogenic studies have identified a plant-specific, ungrouped family of kinesins in which the motor domain does not group to one of the fourteen recognized families. Members of this family contain an N-terminal motor domain, a C-terminal armadillo repeat domain and a conserved destruction box (D-BOX) motif. This domain architecture is unique to plants and to a subset of protists. Further characterization of one representative member from Arabidopsis, Arabidopsis thaliana KINESIN ungrouped clade, gene A (AtKINUa), was completed to ascertain its functional role in plants. Fluorescence confocal microscopy revealed an accumulation of ATKINUA:GFP at the preprophase band (PPB) in a cell cycle-dependent manner in Arabidopsis epidermal cells and tobacco BY-2 cells. Fluorescence accumulation was highest during prophase and decreased after nuclear envelope breakdown. A conserved D-BOX motif was identified through alignment of AtKINU homologous sequences. Mutagenesis work with D-BOX revealed that conserved residues were necessary for the observed degradation pattern of ATKINUA:GFP, as well as the targeted accumulation at the PPB. Overall results suggest that AtKINUa is necessary for normal plant growth and/or development and is likely involved with PPB organization through microtubule association and specific cell cycle regulation. The D-BOX motif may function to bridge microtubule organization with changes that occur during progression through mitosis and may represent a novel regulatory motif in plant microtubule motor proteins.

Original languageEnglish (US)
Pages (from-to)247-258
Number of pages12
JournalCytoskeleton
Volume68
Issue number4
DOIs
StatePublished - Apr 2011

Fingerprint

Kinesin
Arabidopsis
Cell Cycle
Microtubules
Armadillos
Microtubule Proteins
Prophase
Plant Proteins
Terminal Repeat Sequences
Nuclear Envelope
Sequence Homology
Growth and Development
Fluorescence Microscopy
Mitosis
Confocal Microscopy
Mutagenesis
Genes
Tobacco
Fluorescence
Organizations

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Structural Biology

Cite this

Malcos, Jennelle L. ; Cyr, Richard J. / An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner. In: Cytoskeleton. 2011 ; Vol. 68, No. 4. pp. 247-258.
@article{eb795f8c899c45df94273c6e7c88fc05,
title = "An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner",
abstract = "Past phylogenic studies have identified a plant-specific, ungrouped family of kinesins in which the motor domain does not group to one of the fourteen recognized families. Members of this family contain an N-terminal motor domain, a C-terminal armadillo repeat domain and a conserved destruction box (D-BOX) motif. This domain architecture is unique to plants and to a subset of protists. Further characterization of one representative member from Arabidopsis, Arabidopsis thaliana KINESIN ungrouped clade, gene A (AtKINUa), was completed to ascertain its functional role in plants. Fluorescence confocal microscopy revealed an accumulation of ATKINUA:GFP at the preprophase band (PPB) in a cell cycle-dependent manner in Arabidopsis epidermal cells and tobacco BY-2 cells. Fluorescence accumulation was highest during prophase and decreased after nuclear envelope breakdown. A conserved D-BOX motif was identified through alignment of AtKINU homologous sequences. Mutagenesis work with D-BOX revealed that conserved residues were necessary for the observed degradation pattern of ATKINUA:GFP, as well as the targeted accumulation at the PPB. Overall results suggest that AtKINUa is necessary for normal plant growth and/or development and is likely involved with PPB organization through microtubule association and specific cell cycle regulation. The D-BOX motif may function to bridge microtubule organization with changes that occur during progression through mitosis and may represent a novel regulatory motif in plant microtubule motor proteins.",
author = "Malcos, {Jennelle L.} and Cyr, {Richard J.}",
year = "2011",
month = "4",
doi = "10.1002/cm.20508",
language = "English (US)",
volume = "68",
pages = "247--258",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "4",

}

An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner. / Malcos, Jennelle L.; Cyr, Richard J.

In: Cytoskeleton, Vol. 68, No. 4, 04.2011, p. 247-258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner

AU - Malcos, Jennelle L.

AU - Cyr, Richard J.

PY - 2011/4

Y1 - 2011/4

N2 - Past phylogenic studies have identified a plant-specific, ungrouped family of kinesins in which the motor domain does not group to one of the fourteen recognized families. Members of this family contain an N-terminal motor domain, a C-terminal armadillo repeat domain and a conserved destruction box (D-BOX) motif. This domain architecture is unique to plants and to a subset of protists. Further characterization of one representative member from Arabidopsis, Arabidopsis thaliana KINESIN ungrouped clade, gene A (AtKINUa), was completed to ascertain its functional role in plants. Fluorescence confocal microscopy revealed an accumulation of ATKINUA:GFP at the preprophase band (PPB) in a cell cycle-dependent manner in Arabidopsis epidermal cells and tobacco BY-2 cells. Fluorescence accumulation was highest during prophase and decreased after nuclear envelope breakdown. A conserved D-BOX motif was identified through alignment of AtKINU homologous sequences. Mutagenesis work with D-BOX revealed that conserved residues were necessary for the observed degradation pattern of ATKINUA:GFP, as well as the targeted accumulation at the PPB. Overall results suggest that AtKINUa is necessary for normal plant growth and/or development and is likely involved with PPB organization through microtubule association and specific cell cycle regulation. The D-BOX motif may function to bridge microtubule organization with changes that occur during progression through mitosis and may represent a novel regulatory motif in plant microtubule motor proteins.

AB - Past phylogenic studies have identified a plant-specific, ungrouped family of kinesins in which the motor domain does not group to one of the fourteen recognized families. Members of this family contain an N-terminal motor domain, a C-terminal armadillo repeat domain and a conserved destruction box (D-BOX) motif. This domain architecture is unique to plants and to a subset of protists. Further characterization of one representative member from Arabidopsis, Arabidopsis thaliana KINESIN ungrouped clade, gene A (AtKINUa), was completed to ascertain its functional role in plants. Fluorescence confocal microscopy revealed an accumulation of ATKINUA:GFP at the preprophase band (PPB) in a cell cycle-dependent manner in Arabidopsis epidermal cells and tobacco BY-2 cells. Fluorescence accumulation was highest during prophase and decreased after nuclear envelope breakdown. A conserved D-BOX motif was identified through alignment of AtKINU homologous sequences. Mutagenesis work with D-BOX revealed that conserved residues were necessary for the observed degradation pattern of ATKINUA:GFP, as well as the targeted accumulation at the PPB. Overall results suggest that AtKINUa is necessary for normal plant growth and/or development and is likely involved with PPB organization through microtubule association and specific cell cycle regulation. The D-BOX motif may function to bridge microtubule organization with changes that occur during progression through mitosis and may represent a novel regulatory motif in plant microtubule motor proteins.

UR - http://www.scopus.com/inward/record.url?scp=79952807090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952807090&partnerID=8YFLogxK

U2 - 10.1002/cm.20508

DO - 10.1002/cm.20508

M3 - Article

C2 - 21387573

AN - SCOPUS:79952807090

VL - 68

SP - 247

EP - 258

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 4

ER -