Analyses of Cryogenic Samples Using Ion-Induced Desorption and Multiphoton Resonance Ionization

Matthew H. Ervin, Mark C. Wood, Nicholas Winograd

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, Ion-beam-Induced desorption with multiphoton resonance Ionization detection of desorbed neutral molecules Is used to characterize frozen aqueous solutions. This type of matrix Is of particular Importance since It serves as a model for biological matrices. The time-of-flight mass spectrum, obtained In this way for a millimolar tryptophan/H 2 O solution, Is virtually Identical to that for a submonolayer of tryptophan on a silicon wafer. The tryptophan signal from a frozen solution Is demonstrated to have a linear dependence on concentration by using 4,4′-biphenyldiol as an Internal standard. A detection limit of 2 × 10 -6 M Is also demonstrated. Since our Ion beam samples one layer of 0.1 cm 2 and we assume 10 15 molecules/cm 2 of Ice, this concentration corresponds to approximately 4 × 10 6 molecules/layer. It Is also shown that the signal exhibits an exponential decay with primary Ion dose due to the accumulation of primary Ion damage In the near-surface molecules.

Original languageEnglish (US)
Pages (from-to)417-420
Number of pages4
JournalAnalytical Chemistry
Volume65
Issue number4
DOIs
StatePublished - Feb 1 1993

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Cryogenics
Ionization
Desorption
Tryptophan
Ions
Molecules
Ion beams
Ice
Silicon wafers

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

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abstract = "In this study, Ion-beam-Induced desorption with multiphoton resonance Ionization detection of desorbed neutral molecules Is used to characterize frozen aqueous solutions. This type of matrix Is of particular Importance since It serves as a model for biological matrices. The time-of-flight mass spectrum, obtained In this way for a millimolar tryptophan/H 2 O solution, Is virtually Identical to that for a submonolayer of tryptophan on a silicon wafer. The tryptophan signal from a frozen solution Is demonstrated to have a linear dependence on concentration by using 4,4′-biphenyldiol as an Internal standard. A detection limit of 2 × 10 -6 M Is also demonstrated. Since our Ion beam samples one layer of 0.1 cm 2 and we assume 10 15 molecules/cm 2 of Ice, this concentration corresponds to approximately 4 × 10 6 molecules/layer. It Is also shown that the signal exhibits an exponential decay with primary Ion dose due to the accumulation of primary Ion damage In the near-surface molecules.",
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Analyses of Cryogenic Samples Using Ion-Induced Desorption and Multiphoton Resonance Ionization. / Ervin, Matthew H.; Wood, Mark C.; Winograd, Nicholas.

In: Analytical Chemistry, Vol. 65, No. 4, 01.02.1993, p. 417-420.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analyses of Cryogenic Samples Using Ion-Induced Desorption and Multiphoton Resonance Ionization

AU - Ervin, Matthew H.

AU - Wood, Mark C.

AU - Winograd, Nicholas

PY - 1993/2/1

Y1 - 1993/2/1

N2 - In this study, Ion-beam-Induced desorption with multiphoton resonance Ionization detection of desorbed neutral molecules Is used to characterize frozen aqueous solutions. This type of matrix Is of particular Importance since It serves as a model for biological matrices. The time-of-flight mass spectrum, obtained In this way for a millimolar tryptophan/H 2 O solution, Is virtually Identical to that for a submonolayer of tryptophan on a silicon wafer. The tryptophan signal from a frozen solution Is demonstrated to have a linear dependence on concentration by using 4,4′-biphenyldiol as an Internal standard. A detection limit of 2 × 10 -6 M Is also demonstrated. Since our Ion beam samples one layer of 0.1 cm 2 and we assume 10 15 molecules/cm 2 of Ice, this concentration corresponds to approximately 4 × 10 6 molecules/layer. It Is also shown that the signal exhibits an exponential decay with primary Ion dose due to the accumulation of primary Ion damage In the near-surface molecules.

AB - In this study, Ion-beam-Induced desorption with multiphoton resonance Ionization detection of desorbed neutral molecules Is used to characterize frozen aqueous solutions. This type of matrix Is of particular Importance since It serves as a model for biological matrices. The time-of-flight mass spectrum, obtained In this way for a millimolar tryptophan/H 2 O solution, Is virtually Identical to that for a submonolayer of tryptophan on a silicon wafer. The tryptophan signal from a frozen solution Is demonstrated to have a linear dependence on concentration by using 4,4′-biphenyldiol as an Internal standard. A detection limit of 2 × 10 -6 M Is also demonstrated. Since our Ion beam samples one layer of 0.1 cm 2 and we assume 10 15 molecules/cm 2 of Ice, this concentration corresponds to approximately 4 × 10 6 molecules/layer. It Is also shown that the signal exhibits an exponential decay with primary Ion dose due to the accumulation of primary Ion damage In the near-surface molecules.

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