Analyses of RAS Regulation of Eye Development in Drosophila melanogaster

Lucy C. Firth, Wei Li, Hui Zhang, Nicholas E. Baker

Research output: Contribution to journalReview article

19 Citations (Scopus)

Abstract

Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.

Original languageEnglish (US)
Pages (from-to)711-721
Number of pages11
JournalMethods in enzymology
Volume407
DOIs
StatePublished - Jan 1 2006

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Drosophila melanogaster
Cells
Receptor Protein-Tyrosine Kinases
ras Genes
Cellular Structures
Cell Cycle Checkpoints
Drosophila
Cell Differentiation
Cell Survival
Cell Cycle
Cell Death
Assays
Genes
Specifications

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Firth, Lucy C. ; Li, Wei ; Zhang, Hui ; Baker, Nicholas E. / Analyses of RAS Regulation of Eye Development in Drosophila melanogaster. In: Methods in enzymology. 2006 ; Vol. 407. pp. 711-721.
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Analyses of RAS Regulation of Eye Development in Drosophila melanogaster. / Firth, Lucy C.; Li, Wei; Zhang, Hui; Baker, Nicholas E.

In: Methods in enzymology, Vol. 407, 01.01.2006, p. 711-721.

Research output: Contribution to journalReview article

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