Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography

Ken Ichi Harada; Kenji Matsuura; Makoto Suzuki; Hisao Oka; Mariyo F. Watanabe; Shinshi Oishi; Andrew M. Dahlem; Val R. Beasley; Wayne W. Carmichael

doi:10.1016/S0021-9673(01)84589-1

Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography

Ken Ichi Harada, Kenji Matsuura, Makoto Suzuki, Hisao Oka, Mariyo F. Watanabe, Shinshi Oishi, Andrew M. Dahlem, Val R. Beasley, Wayne W. Carmichael

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C₁₈ cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.

Original language	English (US)
Pages (from-to)	275-283
Number of pages	9
Journal	Journal of Chromatography A
Volume	448
Issue number	C
DOIs	https://doi.org/10.1016/S0021-9673(01)84589-1
State	Published - 1988

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(01)84589-1

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title = "Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography",

abstract = "A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C18 cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.",

author = "Harada, {Ken Ichi} and Kenji Matsuura and Makoto Suzuki and Hisao Oka and Watanabe, {Mariyo F.} and Shinshi Oishi and Dahlem, {Andrew M.} and Beasley, {Val R.} and Carmichael, {Wayne W.}",

year = "1988",

doi = "10.1016/S0021-9673(01)84589-1",

language = "English (US)",

volume = "448",

pages = "275--283",

journal = "Journal of Chromatography",

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T1 - Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography

AU - Harada, Ken Ichi

AU - Matsuura, Kenji

AU - Suzuki, Makoto

AU - Oka, Hisao

AU - Watanabe, Mariyo F.

AU - Oishi, Shinshi

AU - Dahlem, Andrew M.

AU - Beasley, Val R.

AU - Carmichael, Wayne W.

PY - 1988

Y1 - 1988

N2 - A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C18 cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.

AB - A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C18 cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.

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U2 - 10.1016/S0021-9673(01)84589-1

DO - 10.1016/S0021-9673(01)84589-1

M3 - Article

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AN - SCOPUS:0023679789

SN - 0021-9673

VL - 448

SP - 275

EP - 283

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - C

ER -

Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography

Abstract

All Science Journal Classification (ASJC) codes

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