Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography

Ken Ichi Harada, Kenji Matsuura, Makoto Suzuki, Hisao Oka, Mariyo F. Watanabe, Shinshi Oishi, Andrew M. Dahlem, Val R. Beasley, Wayne W. Carmichael

Research output: Contribution to journalArticlepeer-review

175 Scopus citations

Abstract

A simple, rapid and reliable chemical analysis method for microcystins (cyanoginosins) has been studied. Three different mobile phases for high-performance liquid chromatography were selected and optimized. Also the adsorptive powers of three commercially available C18 cartridges were compared and the results successfully applied to the clean up of three of the toxins. Finally a total system for the analysis and isolation of microcystins was established.

Original languageEnglish (US)
Pages (from-to)275-283
Number of pages9
JournalJournal of Chromatography A
Volume448
Issue numberC
DOIs
StatePublished - 1988

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Analysis and purification of toxic peptides from cyanobacteria by reversed-phase high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this