Analysis of artificially degraded DNA using STRs and SNPs-results of a collaborative European (EDNAP) exercise

L. A. Dixon, A. E. Dobbins, H. K. Pulker, J. M. Butler, P. M. Vallone, M. D. Coble, W. Parson, B. Berger, P. Grubwieser, H. S. Mogensen, N. Morling, K. Nielsen, J. J. Sanchez, E. Petkovski, A. Carracedo, P. Sanchez-Diz, E. Ramos-Luis, M. Brion, J. A. Irwin, R. S. Just & 7 others O. Loreille, Thomas John Parsons, D. Syndercombe-Court, H. Schmitter, B. Stradmann-Bellinghausen, K. Bender, P. Gill

Research output: Contribution to journalArticle

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Abstract

Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service®, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.

Original languageEnglish (US)
Pages (from-to)33-44
Number of pages12
JournalForensic Science International
Volume164
Issue number1
DOIs
StatePublished - Dec 1 2006

Fingerprint

DNA Fingerprinting
Forensic Sciences
Single Nucleotide Polymorphism
DNA
Blood Stains
Nucleic Acid Databases
Genetic Markers
Saliva
Technology

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Dixon, L. A., Dobbins, A. E., Pulker, H. K., Butler, J. M., Vallone, P. M., Coble, M. D., ... Gill, P. (2006). Analysis of artificially degraded DNA using STRs and SNPs-results of a collaborative European (EDNAP) exercise. Forensic Science International, 164(1), 33-44. https://doi.org/10.1016/j.forsciint.2005.11.011
Dixon, L. A. ; Dobbins, A. E. ; Pulker, H. K. ; Butler, J. M. ; Vallone, P. M. ; Coble, M. D. ; Parson, W. ; Berger, B. ; Grubwieser, P. ; Mogensen, H. S. ; Morling, N. ; Nielsen, K. ; Sanchez, J. J. ; Petkovski, E. ; Carracedo, A. ; Sanchez-Diz, P. ; Ramos-Luis, E. ; Brion, M. ; Irwin, J. A. ; Just, R. S. ; Loreille, O. ; Parsons, Thomas John ; Syndercombe-Court, D. ; Schmitter, H. ; Stradmann-Bellinghausen, B. ; Bender, K. ; Gill, P. / Analysis of artificially degraded DNA using STRs and SNPs-results of a collaborative European (EDNAP) exercise. In: Forensic Science International. 2006 ; Vol. 164, No. 1. pp. 33-44.
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Dixon, LA, Dobbins, AE, Pulker, HK, Butler, JM, Vallone, PM, Coble, MD, Parson, W, Berger, B, Grubwieser, P, Mogensen, HS, Morling, N, Nielsen, K, Sanchez, JJ, Petkovski, E, Carracedo, A, Sanchez-Diz, P, Ramos-Luis, E, Brion, M, Irwin, JA, Just, RS, Loreille, O, Parsons, TJ, Syndercombe-Court, D, Schmitter, H, Stradmann-Bellinghausen, B, Bender, K & Gill, P 2006, 'Analysis of artificially degraded DNA using STRs and SNPs-results of a collaborative European (EDNAP) exercise', Forensic Science International, vol. 164, no. 1, pp. 33-44. https://doi.org/10.1016/j.forsciint.2005.11.011

Analysis of artificially degraded DNA using STRs and SNPs-results of a collaborative European (EDNAP) exercise. / Dixon, L. A.; Dobbins, A. E.; Pulker, H. K.; Butler, J. M.; Vallone, P. M.; Coble, M. D.; Parson, W.; Berger, B.; Grubwieser, P.; Mogensen, H. S.; Morling, N.; Nielsen, K.; Sanchez, J. J.; Petkovski, E.; Carracedo, A.; Sanchez-Diz, P.; Ramos-Luis, E.; Brion, M.; Irwin, J. A.; Just, R. S.; Loreille, O.; Parsons, Thomas John; Syndercombe-Court, D.; Schmitter, H.; Stradmann-Bellinghausen, B.; Bender, K.; Gill, P.

In: Forensic Science International, Vol. 164, No. 1, 01.12.2006, p. 33-44.

Research output: Contribution to journalArticle

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AU - Dixon, L. A.

AU - Dobbins, A. E.

AU - Pulker, H. K.

AU - Butler, J. M.

AU - Vallone, P. M.

AU - Coble, M. D.

AU - Parson, W.

AU - Berger, B.

AU - Grubwieser, P.

AU - Mogensen, H. S.

AU - Morling, N.

AU - Nielsen, K.

AU - Sanchez, J. J.

AU - Petkovski, E.

AU - Carracedo, A.

AU - Sanchez-Diz, P.

AU - Ramos-Luis, E.

AU - Brion, M.

AU - Irwin, J. A.

AU - Just, R. S.

AU - Loreille, O.

AU - Parsons, Thomas John

AU - Syndercombe-Court, D.

AU - Schmitter, H.

AU - Stradmann-Bellinghausen, B.

AU - Bender, K.

AU - Gill, P.

PY - 2006/12/1

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