Analysis of E. coli K5 capsular polysaccharide heparosan

Mellisa Ly, Zhenyu Wang, Tatiana N. Laremore, Fuming Zhang, Weihong Zhong, Dennis Pu, Dmitri V. Zagorevski, Jonathan S. Dordick, Robert J. Linhardt

Research output: Contribution to journalReview articlepeer-review

38 Scopus citations

Abstract

Heparosan is the key precursor for the preparation of bioengineered heparin, a potential replacement for porcine intestinal heparin, an important anticoagulant drug. The molecular weight (MW) distribution of heparosan produced by the fermentation of E. coli K5 was investigated. Large-slab isocratic and mini-slab gradient polyacrylamide gel electrophoresis (PAGE) were used to analyze the MW and polydispersity of heparosan. A preparative method that allowed fractionation by continuous-elution PAGE was used to obtain heparosan MW standards. The MWs of the heparosan standards were determined by electrospray ionization Fourier-transform mass spectrometry (ESI-FT-MS). A ladder of the standards was then used to determine the MW properties of polydisperse heparosan samples. Unbleached and bleached heparosan produced by fermentation of E. coli K5 had similar number-averaged MWs (MN), weight-averaged MWs (M W), and MW ranges of 3,000 to 150,000 Da.

Original languageEnglish (US)
Pages (from-to)737-745
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume399
Issue number2
DOIs
StatePublished - Jan 2011

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Fingerprint Dive into the research topics of 'Analysis of E. coli K5 capsular polysaccharide heparosan'. Together they form a unique fingerprint.

Cite this